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1、基础研究BASICRESEARCH急性胆道梗阻大鼠肝细胞死亡方式的研究徐锋，戴朝六，贾昌俊，许永庆，彭松林，顾玺，赵阳徐锋，戴朝六，贾昌俊，许永庆，彭松林，顾玺，赵阳，中国医科大学盛京医院肝胆外科辽宁省沈阳市110004徐锋，中国医科大学盛京医院肝胆外科讲师，博士，主要从事肝胆疾病基础与临床研究.通讯作者：徐锋，110004，辽宁省沈阳市和平区三好街36号，中国医科大学盛京医院肝胆外科.xufeng_sy@163.com电话：024-83955232收稿日期：接受日期：■背景资料急性胆道梗阻往往导致不同程度的肝功能损伤甚至急性肝功能衰竭，其损伤的确切作用机制尚不清楚.胀亡和凋
2、亡是细胞的两种死亡方式，但目前对急性胆道梗阻时肝细胞的死亡方式仍存在争议.StudyofCelldeathpatternsinratswithacutebileductobstructionFengXU,Chao-liuDAI,Chang-junJIA,Yong-qingXU,Song-linPENG,XiGU,YangZHAOFengXU,Chao-liuDAI,Chang-junJIA,Yong-qingXU,Song-linPENG,XiGU,YangZHAO,DepartmentofHepatobiliarySurgery,ShengjingHospital,Chin
3、aMedicalUniversity,Shenyang110004,LiaoningProvince,ChinaCorrespondenceto:DoctorFengXu,DepartmentofHepatobiliarySurgery,ShengjingHospital,ChinaMedicalUniversity,Shenyang110004,LiaoningProvince,China.xufeng_sy@163.comReceived:Accepted:AbstractAIM:Toinvestigatehepaticcelldeathpatternsinratswit
4、hacutebileductligation.METHODS:MaleWistarratsweredividedrandomlyintobileductligationgroup(BDL,n=5) andcontrolgroup(C,n=5).RatsofBDLgroupunderwentligationanddivisionofcommonbile11ducttosetupacutebileductobstructionmodelaccordingtoYoshidome’smaneuver,butratsofCgrouponlyunderwentdissociationof
5、commonbileduct.Allanimalsweresacrificedonday7.Flowcytometrywasusedtocountthepercentageofhepatocyteapoptosisandoncosis.H&Estainandelectronmicroscopewereusedtoobservehepatocyteapoptosis,oncosisandhistopathologicalchange.Anautoanalyzerwasusedtodetermineserumtotalbilirubin(TBIL),directbilirubin
6、(DBIL),alkalinephosphatase(ALP),glutamyltranspeptidase(γ-GT),alanineaminotransferase(ALT),aspartateaminotransferase(AST)andlactatedehydrogenase(LDH)levels.RESULTS:ThepercentageofcelloncosisornecrosiswashigherinBDLgroupthanthatinCgroup(oncosis:25.41±2.18vs6.12±1.69;necrosis:3.99±1.24vs0.79±0
7、.29;allP<0.05).ThepercentageofoncosiswashigherthanapoptosisinBDLgroup(25.41±2.18vs2.79±1.43).Hepatichistopathologicalchangeshowedbileductproliferation,fibrousconnectivetissueproliferation,hepaticcelloncosisincreasingobviouslyandlivercellcordmorederangeme
