cpg基序重组质粒增强小鼠免疫和抗病力效应

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1、CpG基序重组质粒增强小鼠免疫和抗病力的效应遗传学专业研究生：靳艳指导老师：高荣教授为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个CpG基序的寡聚核苷酸，重组构建含CpG的VRl012质粒(VRlC)：制备壳聚糖纳米颗粒包裹重组质粒(CNP-VRlC)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，SandwichELISA测定血清免疫球蛋白和白细胞介素含量。研究结果表明：CNP-VRlC能显著提高小鼠血清IgG、IgM和IgA含量，促进小鼠血清IL一2、I

2、L一6及IL一4显著增多，同时淋巴细胞和单核细胞数量增加，与对照组小鼠差异显著(P

3、天然免疫，大肠杆菌攻毒EnhancementoftherecombinantplasmidwithCpGmotifsonimmunityandresistanceofmiceMajor：GeneticsMasterCandiate：JinyanTutor：GaorongInordertodevelopasafeandeffectiveimmunoadjuvanttoenhancetheimmunityandresistanceofanimalsagainstinfection，anovelCpGOligodeoxynucleotidescontaining1CpGm

4、otifswassynthesizedandinsertedintotheVRl012plasmid，designatedasVRIC．ThentherecombinantVR1CwasentrappedwithChitosannanoparticles(CNP)preparedbythemethodofionicCROSSlinkage，andemployedtoinjectmuscularly3-weeksoldKunmingmice；theblankVRl012packedwithCNPandsalinewereusedtoinoculatemiceasthe

5、controlgroups．28daysafterinoculation．aIlmicewereorallyfed、Ⅳith0．4ml2x108CFU／permousevirulenthemorrhagicenteritisE．colitochallengetheresistanceagainstinfection．SandwichELISAwasusedtodetectthecontentofimmunoglobulinsandifiterleukinsintheseraofmice．Theresultsfoundthatcomparedwithcontrolmi

6、ce，thecontentofIgG’IgaandlgMremarkablyincreasedintheseraofVRlC—CNPtreatedmice(P<0．05)，SOweretheIL一2．IL一4andIL-6．ThenumberoflymphocytesandmonocyteswerealsoraisedconsiderablyinthebloodofVRIC—CNPgroup(P

7、erentdegreesinVRlC-CNPmicewhichweresignificantlyhigherthanthoseofthecontrolgroupsexcepttheneutrophils；andtheVRICtreatedmiceallsurvivedwithoutsymptomsandlesions；whilethecontrolmicemanifestedevidentsymptomsandlesionsWithdiffusehemorrhageinstomach．smallintestineandperitoneum．Thesedemons