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ID:32048941
大小:5.99 MB
页数:74页
时间:2019-01-31
《多沙唑嗪对映体对大鼠离体肠系膜微动脉药理作用的分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中文摘要的最大收缩效应依次为:Phe=NA=Mox,Clo的最大收缩反应仅为1.07%,Agm和Xyl未引起任何收缩反应;0【受体激动剂的效价强度依次为:NAO.05),Clo与Xvl的最大反应显著增大至33.35%禾n1.74%(P2、三级阻力血管标本的最适前负荷,但是该标准化程序无法适用于大鼠离体肠系膜动脉二级阻力血管标本。2对于大鼠离体肠系膜动脉二级、三级阻力血管标本,(士)Dox阻断0【,.肾上腺素受体的活性相似。(.)Dox对二级阻力血管O【1.肾上腺素受体的阻断作用显著弱于三级阻力血管,而(+)Dox呈现与(-)Dox相反的特征。3大鼠离体肠系膜动脉二级阻力血管多次暴露于Q受体激动剂后,表现出明显的or2_I肾上腺素受体介导的血管收缩反应。关键词:多沙唑嗪对映体;Oil_11肾上腺素受体;(12-II肾上腺素受体;肠系膜微动脉;最适前负荷;大鼠3、英文摘要PharmacologicaleffectsofdoxazosinenantiomersintheratisolatedmesentericresistancearteriesABSTRACTObjective:Toanalysetheblockingeffectsof(一)doxazosin,(+)doxazosinand(+)doxazosinon(11一adrenoceptorsintheratisolatedmesentericresistancearteries,andobservethecharacte4、risticsofVasoconstrictiVeresponsestoagmatine(Agm)and0【一adrenoceptoragonistsintheratisolatedmesentericresistancearteries.Methods:Preparationsofthesecondandthirdgenerationbranchesoftheratmesentericarteryweredissectedandprepared.Cumulativeconcentration-vasoconstricti5、oncurvesfornoradrenaline(NA),phenylephrine(Phe),moxonidine(Mox),c1.onidine(Clo),xylazine(Xyl)andagmatine(Agm)wererecordedusingDMTwiremyographsystem620M.Results:1OptimalpreloaddeterminedinthesecondbranchoftheratmesentericarteryNormalizedpreloadscalculatedbytheDMTno6、rmalizationsoftwarewere3.27士O.95mN(n=32).Under5mNpreload,however,vasoconstrictiveresponsestoPheat3gmol·L~and300gmol·L。1weresignificantlylargerthanthatundernormalizedpreloads(P<0.05andP<0.01).Ontheotherhand,vasoconstrictiveresponsetoPheat3gmol·L‘1wassignificantlysm7、allerinthepreparationsunder10mNpreloadsthanthatundernormalizedpreloads(P<0.05).Under20mNpreload,vasoconstrictiveresponsestoPheat3gmol·L。1,109mol·L‘1and300pmol·L。1weresignificantlysmallerthanthatundernormalizedpreloads俨8、thirdbranchoftheratmesentericarteryNormalizedpreloadscalculatedbytheDMTnormalizationsoftwarewere1.09士O.33mN(n=7).ThevaluesofEm。。and—LogECs0obtainedfromt
2、三级阻力血管标本的最适前负荷,但是该标准化程序无法适用于大鼠离体肠系膜动脉二级阻力血管标本。2对于大鼠离体肠系膜动脉二级、三级阻力血管标本,(士)Dox阻断0【,.肾上腺素受体的活性相似。(.)Dox对二级阻力血管O【1.肾上腺素受体的阻断作用显著弱于三级阻力血管,而(+)Dox呈现与(-)Dox相反的特征。3大鼠离体肠系膜动脉二级阻力血管多次暴露于Q受体激动剂后,表现出明显的or2_I肾上腺素受体介导的血管收缩反应。关键词:多沙唑嗪对映体;Oil_11肾上腺素受体;(12-II肾上腺素受体;肠系膜微动脉;最适前负荷;大鼠
3、英文摘要PharmacologicaleffectsofdoxazosinenantiomersintheratisolatedmesentericresistancearteriesABSTRACTObjective:Toanalysetheblockingeffectsof(一)doxazosin,(+)doxazosinand(+)doxazosinon(11一adrenoceptorsintheratisolatedmesentericresistancearteries,andobservethecharacte
4、risticsofVasoconstrictiVeresponsestoagmatine(Agm)and0【一adrenoceptoragonistsintheratisolatedmesentericresistancearteries.Methods:Preparationsofthesecondandthirdgenerationbranchesoftheratmesentericarteryweredissectedandprepared.Cumulativeconcentration-vasoconstricti
5、oncurvesfornoradrenaline(NA),phenylephrine(Phe),moxonidine(Mox),c1.onidine(Clo),xylazine(Xyl)andagmatine(Agm)wererecordedusingDMTwiremyographsystem620M.Results:1OptimalpreloaddeterminedinthesecondbranchoftheratmesentericarteryNormalizedpreloadscalculatedbytheDMTno
6、rmalizationsoftwarewere3.27士O.95mN(n=32).Under5mNpreload,however,vasoconstrictiveresponsestoPheat3gmol·L~and300gmol·L。1weresignificantlylargerthanthatundernormalizedpreloads(P<0.05andP<0.01).Ontheotherhand,vasoconstrictiveresponsetoPheat3gmol·L‘1wassignificantlysm
7、allerinthepreparationsunder10mNpreloadsthanthatundernormalizedpreloads(P<0.05).Under20mNpreload,vasoconstrictiveresponsestoPheat3gmol·L。1,109mol·L‘1and300pmol·L。1weresignificantlysmallerthanthatundernormalizedpreloads俨8、thirdbranchoftheratmesentericarteryNormalizedpreloadscalculatedbytheDMTnormalizationsoftwarewere1.09士O.33mN(n=7).ThevaluesofEm。。and—LogECs0obtainedfromt
8、thirdbranchoftheratmesentericarteryNormalizedpreloadscalculatedbytheDMTnormalizationsoftwarewere1.09士O.33mN(n=7).ThevaluesofEm。。and—LogECs0obtainedfromt
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