picalm和clu基因多样性对健康青年海马功能连接的影响

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1、天津医科大学硕士学位论文AbstractBackground：nlers385179singlenucleotidepolymorphism(SNP)inthePICALMgeneandrsl136000SNPintheCLUgenearerecentlyassociated、析mAlzheimer’Sdisease(AD)．Investigatingtheeffectsofthesegeneticvariantsontheresting-statefunctionalconnectivity(rsFC)ofthehippocampusmightprovidenewinsightin

2、toADpathogenesis．Inthisstudy,weinvestigatedthemainandinteractioneffectsofthesetwogeneticvariantsonthehippocampalrsFCinhealthyyoungadults．Methods：Atotalof283healthyyoungChineseHanadultsunderwentgenotypingandresting—statefunctionalMRIexaminations．Boththemainandinteractioneffectsofthesetwogeneticv

3、ariants(PICALMandCLU)onthehippocampalrsFCwereanalyzed．1．GenotypingWeextractedgenomicDNAfrom3000plofwholebloodusingtheEZgeneTMBloodgDNAMiniprepKit(BiomigaInc，SanDiego，CA，USA)．Then，wegenotypedthePICALMrs385179andCLUrSl136000ineachsubjectusingthePCRandligationdetectionreaction(LDR)method．2．Allprep

4、rocessingstepswerecarriedoutusingthestatisticalparametricmapping(SPM8，http：／／www．fil．ion．ucl．ac．uk／spm)basedonMatlab．3．Thepreprocessingofhighresolutionstructuraldataincludingsegmentationofgrayandwhitematter，spatialnormalizationandsmoothwitlla4ininfullwidthathalfmaximum；whilethepreprocessingofrs

5、—fMRIdataincludingslicetiming，realignment，spatialnormalizationtotheMNI，resamplingto3x3mm3cubicvoxels，andsmoothedwitha4n31Tlfullwidthathalfmaximum．4．FunctionalconnectivitywascalculatedusingtheDPARSFsoftwarebasedonMatlab；andthersFCdifferenceswereinvestigatedbetweenthetwogenotypeswiththeage，gender

6、andeducationyearsascovariatesofnointerest．5．Voxel-basedmorphometry(VBM)analysisofstructuralMⅪdatawasperformedtoinvestigategroupdifferencesofgreymattervolume．6．MultiplecomparisonswerestatisticallycorrectedbyMonteCarlosimulation．Finally,thecorrectedstatisticalparametermappingWasoverlappedontoMNII

7、II天津医科大学硕士学位论文standardtemplateandthecoordinate，sizeandpeakfvaluesofeachsignificantclusterwererecorded．ResuIts：ThehippocampusshowedpositiversFCwiththedefaultmodenetworkandnegativersFCwiththeffonto．parietalnetwork．RiskPICALMG—allele