免疫球蛋白与共刺激分子在冠心病发病机制中的作用

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1、天津医科大学硕士学位论文AbstractobjectiveToinvestigatefunctionsofimmunoglobulins，CD40／CD40ligandsystemandCD134／CD134ligandsystemonimmunologicmechanismofcoronaryheartdisease(CHD)．Methods42healthymaleadultrabbitsweredividedinto6groupsrandomly．Normalcontrolgroup(N)：fedwithnormalfood．NormalcontrolandCSAg

2、roup(N+CSA、：fedwithnormalfoodandgivenCSA10mg／kg／dfor4weeks．CHDgroup(CHD)：fedwith1．5％cholesteroldietandintervenedwithiliacarteryballooninjuryattheendoffourthweek．CHDandCSAgroup(CHD+CSA)：fedwith1．5％cholesteroldiet．intervenedwithiliacarteryballooninjuryattheendoffourthweekandgivenCSAlOmg／kg／

3、dfor4weeks．Restenosisgroup(RS)：fedwith1．5％cholesteroldiet．intervenedwithiliacarteryballooninjuryattheendsoffourthandeighthweek。RestenosisandCSAgroup(RS+CSA)：fedwith1．5％cholesteroldiet．intervenedwithiliacarteryballooninjuryattheendsoffourthandeighthweekandgivenCSA10mg／kg／dfor4weeks．Allrabb

4、itswerekilledattheendoftwelfthweek．Allrabbitswereweightedatthebeginningandendingofthestudy．Peripheralvenousbloodweretakenbothatthebeginningandendingofthestudytomeasureroutinebloodtests，liverandkidneyfunction，bloodlipids，andimmunoglobulinsincludingIgE，IgM，IgG．Iliacarteriesweretakenoncerabb

5、itswerekilled．Partoftheiliacarterieswereconservedatthetemperatureof-80。C，whichwerethenusedtomeasuremRNAexpressionsofCD40，CD40ligand(CD40L)，CD134andCD134ligand(CD134L)throughreversetranscriptionandreal-timefluorescentquantitativepolymerasechainreactiontechniques．Anotherpartwerepreservedinf

6、ormaldehyde，thenusedforhistologicalobservation(includingneointimalproliferationandvascularremodelingthroughhematoxylinandeosinstaining，andtheratioofpositiveCD40，CD40L，CD134andCD134Lcellsthroughimmunohistochemicalmethods)．DatawereanalyzedthroughSPSSsoftware(version18．0)，P<0．05isofstatistic

7、allysignificantdifference．ResultsUnfortunately1rabbitofRSgroupdiedfrombleedingcausedbyiliacarteryballooninjuryattheendingofeighthweek，SO41rabbitswerefinallyenrolledinthestudy．Therewerenosignificantdifferencesamongthebasaldata．(1)OnlychangesofIll天津医科大学硕士学位论文highdensi