海藻糖联合磷酸盐冷冻保存血小板的实验.研究

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1、DMSO及2％DMSO联合TS相同，维持血小板低渗休克反应、聚集功能及抑制血小板凋亡的效果优于6％DMSO及2％DMSO联合TS，并能较好地维持血小板的超微结构。关键词海藻糖血小板冷冻保存第3页PlateletcryopreservationusingacombinationoftrehaloseandphosphateascryoprotectantsABSTRACTObjeetive：Theaimofthisstudywastoobservetheprotectivefunctionoftrehaloseandphosphateat-80"Conhumanapheresisplatelet

2、s，andtorealizethelong-term(3months)protectivefonctionofthisplateletstoragesolution．Methods：Humanapheresisplateletsweretreatedwithtrehaloseplusphosphate．6％DMSOand2％DMSOplusTS．thenplacedin·80℃refrigerator．Afterfrozenforlweek,lmonthand3months，sampleswerethaw戗Iandanalyzed．Measurementsincludedplateletcou

3、nt,hypotonieshockresponse，ADPinducedaggregation,apoptosisandultrastruetureofplatelets．Results：(1)Theapoptosisrateofplateletsbeforeandafterloadingtrchalosewere(1．3士o．5)％and(1．9±1．1m‘》O．05)，respectively．(2)Theapoptosisrateofplateletstreatedwithtrehalose(withoutphostphate)andla'chaloseplusphosphatew∽(1

4、1．6-a：3．9)％and(6．44-0．9m妒

5、phategroup(尸>o．05)．(4)TheresultsofHSRofplateletstreatedwimtrehaloseplusphosphatefor1week,1monthand3monthswefe(53．5士8．O)％(51．3圭7．8)％and(50．1士8．8)％，respectively．TheresultsofHSRoftrehaloseplusphosphategroupweresignificantlyhigherthan6％DMSOgroupand2％DMSOplusTSgroup(P<0．05)．(5)TheresultsofPAGofplateletst

6、reatedwithtrehaloseplusphosphatefor1week,1monthand3monthswere(34．3士3．o)呃(32．7士3．5mand(31．3士3．O)％，respectively．Thet℃sultsofPAGoftrehaloseplusphosphategroupWU”∞esignificantlyhigherthantheothertwogroups(P<0．05)，(6)Theapoptosisrateof第4页plateletstreatedwithtrehaloseplusphosphatefor1we圮k,lmonthand3monthsW

7、ere(2．8士0．9)％，(6．m眨．2)％and(6．4士0．9)％，respectively．Theapoptosisrateoftrehalosepl璐phosphategroupWCr@significantlylowerthantheothertwogroups(Pm05)．(7)Platcletstreatedwithtrehaloseplusphosphateretainedint