不同基因型新城疫病毒强毒株对鹅和鸡的致病性.研究

《不同基因型新城疫病毒强毒株对鹅和鸡的致病性.研究》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、6扬州大学硕士学位论文above．Asaresult，boththemorbidityandmortalityofthechallengedchickenswas100％．Chickensshowedclinicalsignsandpathologicalalternationssimilartothoseofchickensinfected、vitllviscerotropicstrainsofNDV．ByusingIHCstainingforthedetectionofNDVFprotein，thelocalizationanddistributionoftheviral

2、antigenintissuesfromtheinfectedchickenswassimilartothoseofgeeseinfectedwithisolateofgooseorigin—genotype峨1．wllile，inchickens，therewasmarkedpreferentialviraltropismforthelymphoidaggregatesassociatedwiththegastrointestinal(GI)tractratherthanfornearbyepithelialceils．Pathologicfindingsinassocia

3、tionwithIHCstainingshowedthatthevelogenicNDvirusesof3genotypescausedsystemicillnessinvolvingmanyorgansandtissuesinchickens．ThetransmissibilityofNDvirusesofdifferentorigininchickensandgeesewasinvestigated．Oralandoculonasalinoculationwith103E11350oftwofieldNDVisolatesofJS／I／97／GoandZJ／I／00／Go

4、resultedin75％一100％and50％～100％morbidity,0—25％and25％一50％mortalityintheinfectedgeese，respectively．Transmissionofthetwogoose-originatedNDVisolatesbycontactwithinfectedgeeseandgoosefecesbothresultedininfectionofallchicekenswith100％mortality．Transmissionfrominfectedgeesetochickensoccurredbyaeroso

5、lspread，butnoneofthechickensdieddespiteacquiringinfection．ThereferencechallengestrainF4sEsandvirulentisolateⅪ，2／97／Chchallengecaused100％morbidityand100％mortalityintheinfectedchickens，respectively．TransmissionoftheNDvirusesofchickenoriginfrominfectedchickenstogeesebyeitherfecaloraerosolsprea

6、dresultedininfectionof50％-75％and100％birds，whichshowed0-25％and50％---75％mortality．respectivelyViruseswereisolatedmoreconsistentlyfromcloacalsamplesthanfromtrachealsampleson5daysafterinfection．111eresultshowedthatmutualtransmissioninchickensandgeeseofsomevelogenicNDvirusesofdifferentorigincoul

7、doccurbyeitlleraerosolorfecalcontact．Inthelastexperiment，23day—oldgoslingswereinoculatedwithJS／1／97／GoforthedynasticdistributionoftheFproteinofNDV，throughthesamemutesmentionedabove．111eviralantigencouldbedetectedasearly越4hpostinoculation(PI)．Onday3PIand7