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《人工合成e-选择素对大鼠局灶性脑缺血再灌注损伤脑组织一氧化氮合酶表达的影响与其机制探讨》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、英文摘要人工合成E-选择素对大鼠局灶性脑缺血再灌注损伤脑组织一氧化氮合酶表达的影响及其机制探讨synthesisofE-selectininSDratsandinterventioncansignificantlyreducethevolumeofcerebralinfarctionandbraintissuewatercontent,andthroughpathologicalsectionobservationresults,furtherconfirmedthatthesyntheticE-selectinhaveprotectiveeffectonbraintissue.Keywor
2、ds:suturemethod;cerebralischemiareperfusioninjury;cerebralinfarctionvolume;PathologicalchangePart2TheeffectsofsyntheticE-selectinonratswithfocalcerebralischemiareperfusioninjurynitricoxidesynthaseandserumnitricoxidecontentexpressionObjective:ToinvestigatetheeffectofsyntheticE-selectinoncerebralisch
3、emiareperfusioninjuryinratbrainnitricoxidesynthase(NOS)andplasmanitricoxide(NO)affectthecontentofexpressionanditsmechanismofaction.Methods:SDratswithfocalcerebralischemiareperfusioninjurymodelwasestablishedbyZeaLongasuturemethodimproved,90maleSDratswererandomlydividedintocontrolgroup,modelgroupanda
4、rtificialsynthesisofE-selectinintreatmentgroup,with30ratsineachgroup.Theratswereincerebralischemia2hreperfusion2h,6h,12h,24h,72hfivetimepointsextractedfromserumsamplesandbraintissuespecimens.Usenitratedeterminationofserumnitricoxidecontentandimmunohistochemistryincerebralischemicneuronalnitricoxide
5、synthase(nNOS),induciblenitricoxidesynthase(iNOS)positivecellnumbertoanalyzethestatisticsbyusingSPSS13.0dataacquisition.Result：1.TheNOresults(1)Controlgroup:therewasnosignificantchange.(2)Modelgroup:theNOcontentinserumofperfusion2hhigherthanthatofthecontrolgroup,thecontentofNOinterval6h~24hincrease
6、d,reachedthepeakat24h,72hhasdecreasedbutstillhigherthanthatofthecontrolgroupateachtimepoint,comparedwiththecontrolgroupincreasedsignificantly(P<0.01).(3)Treatmentgroup:thecontentofserumNOchangetrendwiththemodelgroup,statisticaldatalessthanthemodelgroup(P<0.05),wasmorethanthatincontrolgroup(P<0.01).
7、2.TheNOSResults(1)Controlgroup:therewasnosignificantchange.(2)Modelgroup:thenumberofnNOSpositivecellsbegantoexpressat2hafterreperfusion,reachedapeakafter12h,24hbegantodecrease,ateachtimepoint,comparedwithth
