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1、高脂对小鼠Foxp3基因表达的影响王治校1,2，王崇全2，何朝荣2，詹中群2，王俊峰2，李大主1（430021武汉，华中科技大学附属协和医院心血管内科1；442000湖北十堰，郧阳医学院附属太和医院心血管内科2）[摘要]目的研究高脂对小鼠Foxp3基因表达的影响。方法实验动物分为对照组和高脂饮食组。构建小鼠动脉粥样硬化模型，比较不同组小鼠血脂水平、斑块面积和不同器官组织中Foxp3+CD4+CD25+Tregs细胞的数目；利用RT-PCR和Westernblot分析组织中Foxp3基因转录水平和Foxp3蛋白表达水平。结果与对照组比较，高脂饮食组小鼠血脂水平[TG(2.8±0
2、.19)mmol/L、TC(28.2±1.2)mmol/L]明显升高，斑块面积(25044.32±4920.42)μm2显著增加,而不同器官组织中Foxp3+CD4+CD25+Tregs细胞的数目显著减少(P<0.05)，Foxp3基因转录水平(P<0.001)和Foxp3蛋白表达(P<0.05)降低。结论高脂能抑制不同免疫器官Foxp3基因的表达和功能，可能是高脂致动脉粥样硬化的机制之一。[关键词]高血脂；Foxp3基因；动脉粥样硬化；Foxp3+CD4+CD25+Tregs细胞[中图法分类号]R541.4[文献标志码]AEffectsofhyperlipidemiaone
3、xpressionofFOXP3inApoE-knockoutmiceWANGZhi-Xiao1,2,WANGChong-Quan2,HEChao-Rong2,ZHANZhong-Qun2,WANGJun-Feng2,LiDa-Zhu1(DepartmentofCardiology,Taihehospital,YunYangMedicalCollege,ShiyanHubei442000;DepartmentofCardiology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScience&Technolog
4、y,Wuhan430022)[Abstract]ObjectiveTheaimofthisstudywastoexaminetheeffectofhyperlipidemiaonexpressionofFoxp3gene.MethodsAt8weeksofage,16maleApoE−/−micewererandomlydividedintocontrolgroupandhighfatdietgroupofeight.TocomparethelevelofbloodfatinC57BL/6micethroughconstructingthemodelofatheroscler
5、oticplaqueinApoE−/−mice.TheresultingatheroscleroticlesionswereassessedbydeterminingFoxp3genetranscriptlevelsandinvestigatingtheexpressionofFoxp3proteinindifferenttissues.ResultsThelevelofbloodfat(TG(2.8±0.19)mmol/L、TC(28.2±1.2)mmol/L)wasincreasedsignificantly,atthesametime,thenumberandfunct
6、ionofFoxp3+CD4+CD25+Tregs(P<0.05),Foxp3proteinlevels(P<0.05)andFoxp3genetranscriptlevels(P<0.001)indifferenttissueswerelowinthehighfatdietgroupcomparedwiththecontrolgroupgivennormalfood.Theareaofatheroscleroticlesionsinthehighfatdietgroup(25044.32±4920.42)μm2werehigherthan(597.38±305.43)μm2
7、thatinthecontrolgroup.ConclusionsHyperlipidemiacaninhibittheexpressionandfunctionofFoxp3indifferentimmuneorgans,suggestingitmaybeonemechanismofhyperlipidemiaaggravatetheformationofatherosclerosis.[Keywords]hyperlipidemia;Foxp3gene;atherosclerosis;Foxp3hi
