欢迎来到天天文库
浏览记录
ID:30975674
大小:1.26 MB
页数:13页
时间:2019-01-04
《丹参酮iia增效大鼠骨髓源性内皮祖细胞vegf,sdf-1表达》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、丹参酮IIA增效大鼠骨髓源性内皮祖细胞VEGF、SDF-1表达杨彦陈庆伟曹广煜李桂琼(重庆医科大学附属第二医院老年心血管病科,重庆400010)【摘要】目的研究在丹参酮IIA的辅助下,骨髓源性内皮祖细胞(EPCs)VEGF与SDF-1表达是否能增强。方法Percoll密度梯度离心法分离SD大鼠骨髓单个核细胞,血管内皮生长因子(VEGF)、表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)诱导培养,并进行形态学、免疫学、功能学(Dil-acLDL与FITC-UEA-1双荧光染色)鉴定。将鉴定为EPCs的细胞分为单纯EPCs组、EPCs+丹参酮IIA组,另
2、设单纯丹参酮IIA组及对照组(PBS液)。细胞培养第9d,Westernblot与实时荧光定量PCR检测各组VEGF、SDF-1基因与蛋白表达。结果EPCs+丹参酮IIA组VEGF、SDF-1基因与蛋白表达均高于其余各组(p均<0.05)。结论丹参酮IIA可上调大鼠骨髓源性内皮祖细胞VEGF及SDF-1表达,从而可能在缺血环境中更好促进EPCs归巢,更有效促进血管修复与再生。【关键词】骨髓;内皮祖细胞;丹参酮IIA;归巢;血管新生【中图分类号】R541.4【文献标志码】AEfficiencyofratbonemarrow-derivedendothelialp
3、rogenitorcellsonVEGFandSDF-1expressionwiththeaidofTanshinoneIIA.YangYan,ChenQingWei*,CaoGuangYu,LiGuiQiong.DepartmentofGerontology,SecondAffiliatedHospitalofChongqingMedicalUniversity,Chongqing400010,China.【Abstract】ObjectiveToinvestigatetheeffectofratbonemarrow-derived[基金项目]:国家自然科学
4、基金面上项目(编号:30970826)[作者介绍]:杨彦(1984-),女,硕士,研究方向:老年心血管病学,已发表综述两篇(CSCD核心期刊),SCI一篇(第二作者),电话:13608333907,Email:yangyan97_520@yahoo.cn[通讯作者]:陈庆伟,电话:13609414295,Email:chenqwcq@yahoo.com.cnendothelialprogenitorcellsonVEGFexpressionwiththeaidofTanshinoneIIA.MethodsThemononuclearcellswereisola
5、tedfromratfemoralandtibialbonemarrowusingpercolldensitygradientcentrifugation,theninducedwithvascularendothelialgrowthfactor(VEGF)、basicfibroblastgrowthfactor(bFGF)andepidermalgrowthfactor(EGF)fortwoweeks.Theexpressionofcellmarkerswasassessedbyimmunocytochemistry,andtheattachedcells
6、werestainedwithDil-acLDLandFITC-UEA-1.Thenthisstudyincludedfourgroups:TanshinoneIIA,EPCs,EPCs+TanshinoneIIAandcontrolgroups.TheexpressionlevelsofVEGFandSDF-1wasdetectedbyfluorescentqRT-PCRandwesternblot.ResultsComparedwiththeotherthreegroups,EPCs+TanshinoneIIAgrouphadahigherVEGFandS
7、DF-1expression(P<0.05).ConclusionsEPCscanincreaseVEGFandSDF-1expressionwithcombinationofTanshinoneIIA.HomingrateandrepairingefficacyofEPCscanimprovealsowiththeaidofTanshinoneIIA.EPCscanincreaseVEGFandSDF-1expressionininjuredmyocardiumafterhoming,thusimproveangiogenesisefficiently.【K
8、eywords】bonemarrow;
此文档下载收益归作者所有