notch-1对大鼠嗅球成鞘细胞神经生长因子表达的影响

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1、编号201006168刊用形式论著Notch-1对大鼠嗅球成鞘细胞神经生长因子表达的影响卓飞,孙善全，余维华，刘辉，邱国平，杨美（400016重庆,重庆医科大学人体解剖学教研室，神经科学研究中心）[摘要]目的观察Notch-1对嗅球成鞘细胞（olfactoryensheathingcells，OECs）神经生长因子（nervegrowthfactor，NGF）表达的影响。方法原代培养大鼠OECs，用有活性的Notch质粒（NotchICV），无活性的Notch质粒（NotchVK）以及空质粒分别转染OECs，

2、将实验对象分成活性质粒组，非活性质粒组和空白组，应用免疫荧光双标、Westernblot及RT-PCR技术，检测各组OECs中NGF及其mRNA的表达变化。结果转染Notch-1后，OECs中NGF的表达明显增高。免疫荧光双标结果显示，活性质粒组OECs中NGF的表达较非活性质粒组和空白组均明显增高（P<0.05）；RT-PCR结果显示，活性质粒组（0.4961±0.0269）OECs中NGFmRNA的表达均高于非活性质粒组（0.2445±0.0134）及空白组（0.2459±0.0160）（P<0.01）；

3、Westernblot结果显示，活性质粒组（0.8875±0.0166）OECs中NGF的表达均高于非活性质粒组（0.6862±0.0592）和空白组（0.6821±0.0670）（P<0.01）。结论Notch-1转染OECs后，其NGF的表达上调，提示Notch-1能增强OECs中NGF的合成。[关键词]嗅球成鞘细胞；NGF；Notch-1[中图法分类号]R741.05[文献标志码]ATheeffectofNotch-1onNGFexpressionofolfactoryensheathingcellsZ

4、huoFei,SunShanquan,YuWeihua,LiuHui,QiuGuoping,YangMei(DepartmentofAnatomy,InstituteofNeuroscience,ChongqingMedicalUniversity,Chongqing400016,China)[Abstract]ObjectiveObservetheeffectofNotch-1onNGFexpressionofolfactoryensheathingcells.MethodsActiveNotchplasm

5、id(NotchICV)，inactiveNotchplasmid(NotchVK)andemptyplasmidweretransfectedintoOECsofrats.Immunofluorescence、WesternblotandRT-PCRwereusedtodetecttheexpressionchangesofNGFanditsmRNA.ResultsImmunofluorescenceshowstheexpressionofNGFinNotchICVgroupwasnotablyincrea

6、sedcomparedwithNotchVKgroupandcontrolgroup(p<0.05);RT-PCRshowstheexpressionofNGFmRNAinNotchICVgroup(0.4961±0.0269)wasnotablyincreasedcomparedwithNotchVKgroup(0.2445±0.0134)andcontrolgroup(0.2459±0.0160)(P<0.01).WesternblotshowsthattheexpressionofNGFinNotchI

7、CVgroup(0.8875±0.0166)wasnotablyincreasedcomparedwithNotchVKgroup(0.6862±0.0592)andcontrolgroup(0.6821±0.0670)(P<0.01).ConclusionAllresultssuggestthatNotch-1couldimprovethesynthesisofNGFinOECs.[KeyWords]olfactoryensheathingcells;NGF;Notch-1SupportedbytheNat

8、ionalNaturalScienceFoundationofChina(30670743)andtheNationalNaturalScienceFoundationofChina(CSTC，2008BB5224).Correspondingauthor:SunShanquan,Tel:86-23-68485763,E-mail:sunsq2151@sohu.com编号201006168刊用形式论