嗅成鞘细胞移植对ad大鼠脑内cox活性和线粒体超微结构的影响

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1、嗅成鞘细胞移植对AD大鼠脑内COX活性和线粒体超微结构的影响作者：姚柏春孙天敏王金勇李勇王配军李文春【摘要】目的探讨嗅成鞘细胞(OECs)移植对阿尔茨海默病(AD)大鼠脑内COX活性和线粒体超微结构的影响。方法SD大鼠双侧海马注射Aβ1～40，建立AD大鼠模型。实验动物分为四组：正常对照组、AD模型组、OECs移植组、人工脑脊液(MCSF)注射组，每组12只。体外原代培养OECs并将其移植至AD大鼠双侧海马。运用行为学测试、组织化学、原位杂交结合图像分析以及电镜等技术和工具，观察、比较各组大鼠学习记忆能力、海马CA1区线粒

2、体细胞色素氧化酶（COX）活性、细胞色素氧化酶Ⅱ型亚基(COⅡ)mRNA表达，以及CA1区神经元线粒体超微结构等指标的变化。结果与正常对照组比较，AD模型组大鼠空间学习记忆能力、海马CA1区线粒体COX活性及COⅡmRNA表达明显降低(P<0.05)，线粒体数量减少，结构不清、肿胀、空泡样变、嵴断裂；OECs移植明显上调上述指标(P<0.05)，并对线粒体超微结构有明显的保护作用。结论OECs移植通过改善AD大鼠学习记忆能力、提高海马COX活性和COⅡmRNA表达以及保护线粒体等作用，对大鼠AD具有明显的治疗效

3、果。【关键词】阿尔茨海默病；细胞色素氧化酶；线粒体；嗅成鞘细胞；移植14【Abstract】ObjectiveToobservetheeffectofolfactoryensheathingcells(OECs)transplantationoncytochromeoxidase(COX)andtheultrastructureofhippocampalneuronsmitochondrioninA1zheimer′sdisease(AD)rats.MethodsADmodelwasestablishedbyAβ1～40i

4、njectedintohippocampusCA1.Theratswererandomlydividedintonormalcontrol,model,OECstransplantationandthemanpowercerebralspinalfluid(MCSF)injectedgroups.Therewere12ratseachgroup.OECswereculturedinvitroandinjectedintoADrat′shippocampus.Theabilityoflearningandmemorywasv

5、erifiedbytheperformanceofMorriswatermazetask.TheactivityofCOXinrat′shippocampusCA1wasdetectedwiththemethodofenzymehistochemicalstainingcoupledwithcomputerimageanalysissystem.TheexpressionofCOⅡmRNAwasobservedbyinsituhybridizajtion.Ultrastructuralchangesofneuronmito

6、chondrioninrat′shippocampusCA1wereviewedundertransmissonelectronmicroscope.ResultsComparedwiththenormalcontrolgroup,thelearningandmemoryabilityofAβinjectedratswerelower,thenumberandultrastructureofhippocampusneuronmitochrondrionhaddistinctchange,theactivityofCOXa

7、ndtheexpressionofCOⅡmRNAinhippocampus14CA1oftheanimalsmodelofADhadbeenmarklydeclined.OECstransplantationcouldimproveaboveindexes.ConclusionsTheefficacyofOECsonADmaybepartlyresultfromimprovingtheabilitiesofspatial1earningmemory,theactivitiesofCOX,theexpressionofCOⅡ

8、mRNAandprotectinghippocampusneuronmitochrondrion.【Keywords】Alzheimer′sdisease;Cytochromeoxidase;Mitochondrion;Olfactoryensheathingcells;Transplantation