生化分析仪试剂问交叉污染对总胆汁酸测定的影响及分析

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1、•论著・臨秦氏学嬪早妙。铲樽皿鬆3働生化分析仪试剂间交叉污染对总胆汁酸测定的影响及分析易向民，郑敏（暨南大学医学院附属广州市红十字会医院，广东广州510220）【摘要】目的探讨试剂间交叉污染对日立7600-010全自动生化分析仪演•，根据交叉馬集顼副織眩翠痢繍奄氏眩綁林魁肴柯hl诚匣呛j懑感觸禪柚糖沛蛋门试剂对总胆汁酸测定有干扰影响「影悔。分析总胆固礴St剂砒胆泮酸测國約予瘵報响采厳矽圄国唱瞩固斑锻M嗣旳汁酸的顺序，并車复十次.以确定总丿1H固总卿固醇对总职汁截徇軫喻程此細嘛呦懈m塔删柄丽擴常轴験潮弼购枷虬确定总胆

2、同醇试剂的「扰因素。丽I似删1删味清洗程序，观察仪器特殊清洗程序排除试剂间干扰的效果。结果与单独测定’[对胆汁酸测宛有爾剧ft豆桂・龟胭圜轄删J脚齡疲納前髒俯湖财湃M聊紘引起总胆汁酸测左结果明显升离的上滴帕删为题斛稱畴K俩谢，御瘢I确懈翊劇婀補陥變贿能。纟繩飭卅册取曲御臟桝真荐疽盟爵的年懿，牡洙塞铀竣數比诵潮弱南敢噸月并麻旌阀M陪缰舍奔餐臨：i嘩剛n舸&删诉预料个硬爭用磺涵翩赦瞬清洗吸样针，遽免您克畅"I生。【关霞词】总胆固醇试剂；总胆汁酸；交叉污染中图分类号：R446.1文献标识码：A宴章编号：：1674-465

3、9(2010)03-0021-03InfluenceofBiochemicalAnalyzerCross-contaminationbetweenReagentsonDeterminationofTotalBileAddsYIXiangmuKZHENGMin(GuangzhouRedCrossHospitalofMedicalCollegeofJinanUniversity,Guangzhou510220,China)(Abstract]ObjectiveTostudytheinfluenceoftheTotalC

4、holesterol(TCHOL)reagentsonthedeterminationresultsofTotalBileAcids(TBA)byHitachi7600-010automaticbiochemicalanalyzer.MethodsMixedserumprepared,screenedouttheinterferenceitemsofthedeterminationofTBAaccordingtotheprojectofcross-contaminationselectionprocedures;D

5、efinedtheTCHOLwhichwouldhavesomeinfluenceonthedeterminationofTBA.DeterminedthreetimesofTBAafterTCHOLmeasured,andrepeated10timesinordertodetenninetheimpactofTCHOLonTBA・Low,mediumandhighconcentrationsofTBAweredeterminedafterTCHOLtodefineinfluenceamongthedifferen

6、tconcentrationsof1^A・Reagent1and2ofTCHOLwereaddedtomixedsenim,tomakesurewhichreagentwouldhavecross-interferencewithTBA;Specialcleaningproceduresofequipmentcouldexcludetheeffectofinterferencebetweenthereagents.ResultsComparedwiththegroupofdeterminationofTBAin

7、dependently,thereagentsofTCHOLhadapositiveinterferenceonTheinterferenceonlowconcentrationofTBAwasmoreobviousthanthatonthehighone.Reagent1ofTCHOLcouldsignificantlyincreasethedetermina・tionresultsofTBA,usinglyetoenhancecleaningagentscouldsignificantlyreducethecr

8、oss-contaminationofTCHOLConclusionThereagentsofTCHOLhaveasignificantpositiveinterferenceonthedeterminationofTBA.Whensettingtheanalysisparameters,theordershouldbepaidattentionto,and