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1、3-大豆昔元磺酸钠对氧化低密度脂蛋白致内皮细胞损伤的保护作用研究Sodium3-soyasodiumsulfonateistheinnerskinoftheLDLProtectiveeffectofcellinjuryTheauthor:jingjing,huangyuping,huangyushan,wangshu-people【abstract】objectivetostudy3J-soybeansulfonicacidsodium(DSS)toinduceumbilicalveininoxidizedlow-densitylipoproteinTheprotectivee
2、ffectofendothelialcel1injuryanditsmochanism・MethodstheendothelialcellsofthenavelveinwereculturedinvitroInthecontrolgroup,thecontrolgroup(ox-ldl)andoxidativedamagewereaddedtothevitaminEcontrolgroup(VE+ox-ldl),oxidativedamageisaddedtoDSSlow,middle,highconccntrationgroup(dss-1,dss-manddss-h)Gro
3、up).Theeffectoftheoxy-ldleffectontheendothelialcellsthatpreloadedtheVEandthedifferentconcentrationsofDSSto24h,Continuctotrain24h,MTTmethoddetectscellvitality,colorimetrydetectspropylenedialdehyde(MDA)andnitricoxide(NO)Thecontentoftheenzymelactatedehydrogenase(LDH),superoxidedismutase(SOD),ni
4、tricoxidesynthase(NOS)andGlutathioneperoxidase(gsh-px)activity・Theresultsarepre-addedtotheDSS,whichhasahighconcentrationThevitalityofthedamagedendothelialcellscansignificantlyreducethereleaseofMDAandNO,whichdamageendothelialcellsTheactivityofLDH,NOS,SOD,andgsh-pxofdamagedcellswasenhaneed,but
5、lowconcentrationsofDSSwereusedThestandardhaslittleeffect・ConclusionDSScanreducetheoxidativedamageofoxandLDLtovascularskincellsOxidativeprotection.[keywords]3'-soysulfonate;LDL・Theendothelialcells;ProtectiveeffectAbstract:ObjectiveToinvestigatetheprotectiveeffectandMechanismsof3'-daidzeinsulf
6、onatesodiumonvascularendothelialCellinjuryinducedbyoxidizedlowdensitylipoprotein,MethodsHumanUmbilicalveinendothelialcells(HUVECs)wereculturedinvitroandDividedintofivegroups:control,ox,LDL,dss-1.dss-mandDSS-Hgroups.Endothelialcellswereincubatedwithox-LDLfor24HoursaftertheywereincubatedwithVi
7、taminE(VE)anddifferentTheproliferationofHUVECswasassayedbyThe3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny1tetrazoliurabromideMethod,andthelevelsofNOandMDA,theactivitiesofLDH,SOD,NOSTheyweremeasuredbythelevelofMDAandtheactivityoftheactivityLDHinoxy-ldld
