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1、Genomic组织和染色体的小鼠附睾视黄酸结合蛋白基因(ME-RABP)定位。Abstract文摘Themurineepididymalretinoicacid-bindingprotein(mE-RABP)isspecificallysynthesizedinthemousemid/distalcaputepididymidisandsecretedinthelumen.鼠类附睾的视黄酸结合蛋白(mE-RABP)是专门合成在老鼠中/远端头epididymidis和中分泌腔。Inthisreport,wehavedemonstratedbySouthernblotanalysis
2、ofgenomicDNAthatmE-RABPisencodedbyasingle-copygene.在这篇报告中,我们已经演示了由南方污点,mE-RABP基因组DNA的分析是由基因进行编码的单份。Amouse129/SvJgenomicbacterialartificialchromosome(BAC)librarywasscreenedusingacDNAencodingtheminorformofmE-RABP.一只老鼠129/SvJ基因组的细菌人工染色体(BAC)库是使用一个cDNA筛选编码小mE-RABP形式。OnepositiveBACclonewascharacte
3、rizedandsequencedtodeterminethenucleotidesequenceoftheentiremE-RABPgene.一个积极的BAC克隆特点和测序来确定整个mE-RABP核苷酸序列的基因。ThemolecularcloningofthemE-RABPgenecompletesthecharacterizationofthe20.5-kDa-predictedpreproteinleadingtotheminorandmajorformsofmE-RABP.分子克隆的mE-RABP基因完成描述20.5负责预测preprotein导致轻微和主要形式的mE-
4、RABP。ComparisonoftheDNAsequenceofthepromoterandcodingregionswiththatoftheratepididymalsecretoryproteinI(ESPI)geneshowedthatthemE-RABPgeneistheorthologueoftheESPIgenethatencodesaratepididymalretinoicacid-bindingprotein.比较DNA序列的发起人和编码区域与鼠附睾的分泌蛋白我(ESP我)基因表明,mE-RABP基因是orthologue的ESP我的编码基因鼠附睾的视黄酸结
5、合蛋白。Severalregulatoryelements,includingaputativeandrogenreceptorbindingsite,"CACCC-boxes,"NF-1,Oct-1,andSP-1recognitionsites,areconservedintheproximalpromoter.一些监管元素,包括一个假定的雄激素受体结合位点,“CACCC-boxes,“nf1,10月1日,sp1识别网站,是守恒的近端启动子。AnalysisofthenucleotidesequenceofthemE-RABPgenerevealedthepresenceof
6、sevenexonsandshowedthatthegenomicorganizationishighlyrelatedtoothergenesencodinglipocalins.分析mE-RABP核苷酸序列的基因显示存在七个外显子和显示,基因组织是高度相关的其他基因编码lipocalins。ThemE-RABPgenewasmappedbyfluorescentinsituhybridizationtothe[A3-B]regionofthemurinechromosome2.mE-RABP的基因所绘制荧光原位杂交(a3b]区域的小鼠染色体2。Ourdata,combined
7、withthatofothers,suggestthattheproximalsegmentofthemousechromosome2maybearichregionforgenesencodinglipocalinswithagenomicorganizationhighlyrelatedtothemE-RABPgene.我们的数据,加上其他人的,表明近段鼠标染色体2可能是一个富裕的地区lipocalins基因编码与基因组组织高度相关的mE-RABP基因。Structurean