灯盏花素对缺血再灌注大鼠心肌超微结构及细胞凋亡的影响

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1、灯盏花素对缺血再灌注大鼠心肌超微结构及细胞凋亡的影响龚明玉1，张力2，杜超1，周晓慧1（1承德医学院，河北承德067000；2承德医学院附属医院放疗科，河北承德067000）［摘要］目的：研究灯盏花素(breviscapine)对在体大鼠缺血再灌注损伤的保护作用及其机制。方法：40只SD大鼠随机分为假手术组，缺血再灌注组（IR组），灯盏花素组。灯盏花素组大鼠于术前1周分别腹腔注射不同剂量的灯盏花素（25，50mg.kg-1.d-1），另2组给等量生理盐水。以左冠状动脉前降支穿线结扎法制备心肌缺血模型，松开结扎线造成再灌注。采用Annexinv-PI双染，流式细胞仪检测心肌细

2、胞的凋亡情况；电镜观察各组大鼠心肌超微结构的变化。结果：灯盏花素可明显降低缺血再灌注大鼠心肌细胞的凋亡率（P＜0.05，P＜0.01），且较IR组心肌细胞超微结构损伤程度减轻。结论：灯盏花素对缺血再灌注心肌细胞具有保护作用，其机制可能是通过抑制心肌细胞凋亡来实现的。【关键词】灯盏花素；细胞凋亡；心肌；缺血再灌注；超微结构EffectofBreviscapineAgainstMyocardialIschemiaReperfusiononMyocardialCellUltrastructureandApoptosisinRatsGONGMing-yu，ZHANGLi，DUCha

3、o，etal（1ChengdeMedicalCollege，HebeiChengde067000,China2DepartmentofRsdiationOncology,AffiliatedHospitalofChengdeMedicalCollege,HebeiChengde067000,China）ABSTRACT:Objective:Toexploretheeffectandmechanismofbreviscapineagainstmyocardialischemiareperfusioninjuryinrats.Methods:40SDratsweredivide

4、dintoshamoperationgroup,ischemiareperfusiongroupandbreviscapinegrouprandomly.Ratsinbreviscapinegroupwereinjectedwithbreviscapine25、50mg.kg1.d-1respectivelypriortoischemiareperfusion.othertwogroupsweregivenNSinsteadofbreviscapineequallyataweekbeforetheoperation.Theischemiareperfusionheartmo

5、delwasestablishedbyligatingtheleftanteriordescendingbranchofcoronaryartery.ApoptosisofmyocyteswasdetectedafteroperationinallgroupsbyflowcytometrywithAnnexinⅤ-EGFPandpropidiumiodiode(PI)staining.Theultrastructuralalterationswereobservedunderthetransmissionelectronmicroscopy.Results:brevisca

6、pinecoulddecreasetheoccurrencerateofmyocardialapoptosisduringischemiareperfusioninjuryobviously（P＜0.05，P＜0.01）;Thedegreeofinjuringonultrastructuresofmyocardialcellinbreviscapinegroupisratheralleviatedthaninischemiareperfusiongroup.Conclusion:breviscapinemayprotectmyocardialcellsduringische

7、miareperfusioninjuryandtheprotectivemechanismmaybeobtainedbyinhibitingmyocardialapoptosis.KEYWORDS　breviscapine;apoptosis;Cardiomyocyte;ischemiareperfusion;myocardiacultrastructures心肌缺血再灌注可导致心肌细胞死亡,凋亡在心肌细胞死亡中发挥了重要的作用[1-2]　。细胞凋亡的多少决定着缺血再灌注心肌损伤的严重程度，有效抑制细胞凋亡的发生和