裂殖壶菌schizochytrium spfju512在温度胁迫下的差异蛋白质组学研究

《裂殖壶菌schizochytrium spfju512在温度胁迫下的差异蛋白质组学研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、AbstractAbstractTemperaturewasoneofimportantfactorsaffectcellgrowth，lipidcontentandfattyacidcomposition．ChangingtheratioofunsaturatedfattyacidsinbiologicallipidWaSoneoftheimportantmechanismsfortolerancetolowtemperaturestress．Organismsunderlowtemperaturestress，usuallyincreas

2、edtheunsaturatedfattyacidsorimprovedtheproportionofbranched—chainfattyacidswithinvarioustypesofcellmembraneto～?●lenhancethemembranefluidity,thusensuringthemembranetocarryoutnormalphysiologicalprocesses，suchasmaterialtransport,energyconversion，informationmetabolismandmetabol

3、icregulation．Manyarticlesreportedthatinavarietyoforganisms，thedesaturatse，animportantenzymeofpolyunsaturatedfattyacidsynthesisintheclassicway,wastemperature-sensitivefactorsobviously．amarinelowerfungi，withasimple，fastgrowth，simplefattyacidcompositionandeasytop嘶fy,wastheidea

4、lDHAfermentationbacteria．Byshakeflaskfermentation，acid-heatextractionandGC-MSdetectionanalysisoflipidshowedthatincubationtemperatureWasoneoftheimportantenvironmentalfactorsaffecttheSchizochytriumbiomass，lipidcontentandfattyacidcomposition．Schizochytrium．spFJU·512couldgrowbe

5、tween15。Cand37。C．At15。C，theDHAcontentofSchizochytriummorethan60％，butbiomassandoilyieldfarlessthanothertemperatures．Schizochytriumgrowbetterbetween20。Cand30。C，withahigherlipidcontentandrelativelystableDHAcontent．Whentemperatureroseto370C，biomass，lipidcontentandDHAcontentwere

6、decreased．Totalsaturatedfattyacidsachievedthelli曲estat37。C，thelowestat15。C，between20。Cand30。Ctomaintainstable；totalunsaturatedfattyacidhada150C，minimumat370C，between200Cand300Ctomaintainconstant．Forthefirsttimeusingdifferentialproteomicstoinvestigatethemolecularmechanismofh

7、owtemperatureregulateSchizochytrium，whichpromotedtodeeplyunderstandlipidmetabolismofSchizochytrium．Andthroughexploringtheappropriatesamplepreparationmethodandtwo-dimensionalelectrophoresisconditions，asuitablesystemoftwo-dimensionalelectrophoresistoseparatesolubleproteinsofS

8、chizoch)，triumIII福建师范大学刘静硕士学位论文wassuccessfullyestablished．Finallyputthemethodglass