n注入钝齿棒杆菌as1.998原生质体选育lile高产菌株

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1、郑州大学硕士学位论文Abstractrotation一flaskexPeriments.5.TheeffectsofseedmediumcomPositionsandfermentionmediumeomPositionsontheL一IsoleucineProduetioninflaskwereeonsidered，usingsinglefactoranal}产515methodandorthogonalityexPeriments，wegettheoPtimalmediumofDN一21.IntheoPtimalmedium，theL一isoleu

2、cineyieldofDN一21was8.939/L.Ke)rW0rds:L一Isoleueine，ionbeamimPlanting，ProtoPlast，growtheirelebioassay，imProvedaPerehromatography一sPeetroPhotome饰，orthogonaldesign111郑州大学硕士学位论文目录目录摘要.....................................................................................................

3、..........................……IABSTRACT.....................................……，，…，二，.…，，二，二，..…，.，..........................................……n目录.......……、..................................................................................................................……IV引一言.....

4、.............................................................................................................................……1一文献综述.........................................................................................................……，..……21离子注入技术及在微生物育种领域的应用..............

5、............................................……21.1离子注入技术概述.......……，.............................................................................……21.2离子注入技术的作用机理...............................................................................……21.3离子注入技术在微生物领域的应用实例.…，...........…

6、…，................................……42原生质体诱变技术在微生物育种中的应用..........................................................……72.1原生质体诱变技术概述..……，...........................................................................……72.2原生质体诱变在工业微生物菌种选育中的应用...........................................

7、.……72.3原生质体诱变在工业微生物育种中的应用前景.........................................……103.L一异亮氨酸的应用、测定及生产.................……，................................................……103.IL一异亮氨酸概述.........……，..............................................................................……103.2L一异亮氨酸的结构及

8、理化性质...........................................