nfkb在腰5脊神经结扎引起的神经病理性疼痛中的作用

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1、郑州大学2008届硕!:学位论文英文摘要TheroleofNF一kBintheneuroPathicPaininducedbyLSsPinalnerveligationPostgraduateCandidateZhaiCaihongSuPervis.rXuJitianMediealCollegeofZhengzhouUniversityZhengzhou，450052，P.R.ChinaAbstraCtBaekgroundCentralandPeriPheralnerveinjuryorinflammationoftenleadstoneur

2、oPathiePain，which15charaeterizedbyh邓eralgesia，allodyniaandsPontaneousPain.AlthoughamountofstudieshavebeenPerformedinthePast，theunderlyingmechanismstillremainlargelyunknown.ReeentstudiesindieatethatWalleriandegenerationfollowingnerveinjuryeontributedtothedeveloPmentofneuroPa

3、thicPalnviaProduetionofeytokins.Amongthem，tumorneerosisfaetor一alPha(TNF一o)andinterieukin一l(IL一l)aPPearstoPlayanimPortantroleintheinduetionandmaintenaneeoftheneuroPathiePainafternerveinjury.Previousstudieshavealsoshownthatthesynthesisoftheseeytokinesaswellastheiraetiontotarg

4、eteellsneedtheactivationofnuelearfaetor一kaPPaB(NF一kB)signalPathway.But，theroleofNF一kBintheneuroPathiePain15stillshortofasystemiedata.objeetiveInthePresentstudy，weligatedthelumbar5sPinalnerve(LSSNL)toindueeneuroPathiePain，andthenobservedtheroleofNF一kBinthedeveloPmentoftheneu

5、roPathiePainbyintratheealorintraPeritonealinjectionofPDTC，aPotentinhibitorofNF一kBaetivation.TofurthereonfirmtheaetivationofNF一kapPaBandtheeelltyPeoftheaetivatedNF一kBinLSandL4dorsalrootganglia(DRG)，immunohistoehemistryanddoubleimmunofluoreseeneestainingwerealsoemPloyedinthis

6、study.川子郑州大学2008届硕l:学位论文英文摘要MethodSAdultmaleSPragUe一Dawley(SD)ratsarechoseninthePresentstudy.Firstly，usingbehavioraltests，wesystemicallyobservedtheroleofNF一比inthedeveloPmentoftheneuroPathiePainl，yintrathecalorintraPeritoneainjeetionofdifferentdosesofPDTC(aPotentinhibitorofN

7、F一kBaetivation)indifl七renttimePointbefororafterLSSNL.Then，usingthemethodsofimmunofluoreseentstaining，theaetivationofNF一kaPPaBinLSandL4dorsalrootgangliawasexaminedindifl七renttimePointafterLSSNL.Tofurthereonfirmtheeellt冲eoftheaetivatedNF一kB，doubleimmunofluoreseencestainingwer

8、ealsoemPloyedinthisstudy.Themethodsofbehavioralte、tswerePerformedasfollowing:50%Me