衰变加速因子在神经病理性疼痛中的作用.pdf

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1、·460·现代中西医结合杂志ModernJournalofIntegratedTraditionalChineseandWesternMedicine2014Feb，23(5)衰变加速因子在神经病理性疼痛中的作用朱海娟，王金保(解放军白求恩国际和平医院，河北石家庄050082)[摘要]目的观察衰变加速因子(DAF)在2种外周神经损伤模型大鼠脊髓背角的表达情况，旨在探索它在神经病理性疼痛(NPP)发病机制中的作用。方法将6O只健康雄性SD大鼠分为假手术组、CCI组和SNI组3组，按分组制作模型，分别于术前及术后l，3，7d，用热刺激和机械刺激法测定大鼠的病阈值变化，评估其可

2、靠性后，于术后7d处死大鼠，取L一段脊髓背角，用Western—blot和免疫组化两种技术测定其DAF蛋白含量。结果CCI组和SNI组大鼠于术后l，3，7d痛闽值降低，与假手术组比较有显著性差异，表明模型制作成功。Western—blot检测显示，3组模型大鼠脊髓背角DAF表达发生不同程度降低，CCI组和SNI组与假手术组相比有显著性差异。免疫组化染色显示：CCI组、SNI组大鼠脊髓背角DAF阳性细胞较假手术组显著减少。结论NPP大鼠脊髓背角CD55蛋白表达下降，可能是此处发生补体级联反应的重要原因，CD55在NPP的形成中发挥作用。[关键词]CD55蛋白；神经病理性疼痛

3、；大鼠；衰变加速因子doi：l0．3969／j．issn．1008—8849．2014．05．002[中图分类号]R一332[文献标识码]A[文章编号]1008—8849(2014)05—0460—03EffectofdecayacceleratingfactoronneuropathicpainZhuHaijuan，WangJinbao(BethuneInternationalPeaceHospitalofPLA，Shijiazhuang050082，Hebei，China)Abstract：0bjectiveItistoobservetheexpressionofde

4、cayacceleratingfactor(DAF)inspinalcordoftwoperipheralnerveinjurymodelrats，andinvestigateitsactiononthepathologicalmechanismofneuropathicpain．MethodsSixtyhealthymaleSprague—Dawleyratsweredividedrandomlyintosham—operationgroup，CCIgroupandSNIgroup．Animalmodelsweremadeaccordingtothegroup．Pain

5、thresholdbythermalandmechanicalstimulationwererecordedatpre—operationand1，3，7daysafteroperation，whilethelevelofDAFCD55proteininspinalcordwasdetectedbywestern—blotandimmunohistochem—icalmethod．ResultsPainthresholdbythermalandmechanicalstimulationwerereducedobviouslyat1．3．7daysafteropera—ti

6、oninCCIgroupandSNIgroup，butitwasnotexhibitedobviouslyinsham—operationgroup．Whileinwestern—blot，comparedtosham—operationgroup，theexpressionsofCD55proteininspinalcordwereshoweddown—regulationinCCIgroupandSNIgroup．Inimmunohistochemicalmethod，theexpressionsinspinalcordweremarkedlydecreasedinC

7、CIgroupandSNIgroupthanthatinsham—operation．ConclusionTheCD55proteinmayplayakeyroleonabnormalcomplementactivationinspinalcord，whichexplorestherelationbetweenthechangeofCD55proteinandneuropathicpain．Keywords：CD55protein；neuropathicpain；rat；decayacceleratingfactor前期研究证