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ID:28563209
大小:7.47 MB
页数:42页
时间:2018-12-11
《crmasa检测外周血kras基因点突变和ca199 ca242联合检测在胰腺癌诊断中的作用》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、PCR-MASA检测外周血K-ras基因点突变和CAl9.9、CA242联合检测在胰腺癌诊断中的作用摘要目的:研究联合检测外周血K—ras基因突变和CAl9.9、CA242在胰腺癌诊断中的作用。方法:选择12胰腺癌和同期的17非胰腺癌病人,采用突变等位基因特异性扩增(PCR.MASA)检测胰腺癌患者外周血K.ras基因12位点突变。电化学发光法(ECLIA)检测CAl9—9、CA242。统计分析检测结果。结果:胰腺癌患者外周血K.raS基因12位点突变率为75%(9/12),非胰腺癌组K.ras基因12位点突变率为5.88%(1/17);K.raS基因突变检测
2、诊断胰腺癌的敏感性、特异性分别为75.00%和94.12%;CAl9.9联合K.raS基因突变检测平行实验可以提高诊断胰腺癌的敏感度至83.33%。CAl9-9、CA242和k-ras基因突变与患者性别、年龄、肿瘤大小、部位及肿瘤TNM分期无关(P>0.05)。结论:(1)联合检测胰腺癌患者外周血K-ras基因突变和CAl9-9比单一检测k-ras基因突变提高了诊断敏感度,有利于胰腺癌的诊断。(2)胰腺癌组中k-ras基因突变、CAl9-9、CA242是否升高与患者性别、年龄、肿瘤大小、部位及肿瘤TNM分期无关。(3)外周血CA242升高在胰腺癌组与非胰腺癌组
3、无明显统计学意义,有待进一步研究。(4)PCR-MASA方法简捷、特异、敏感,扩增产物只需常规电泳、染色即可观察结果,无需酶切、杂交、放射性和非放射性显影。硕士研究生:吴昌亮(肝胆外科)指导教师:邱法波教授关键词:胰腺癌;K.ras基因;CAl9-9;CA242;PCR_MASAAbstractObjective:ToevaluatetheclinicalsignificanceofdetectingK_RasgenepointmutationandCAl9.9,CA242testinperipheralbloodofpatientswithpancreati
4、ccal"clnoma·Methods:Inmesameperiod12patientswithpancreaticcarcinomaand1/non.paI:Icreaticcarcinoma,westudiedK.msgene12pointmutationbyPCR·MASA(ml】tantallelespecificamplification)anddetectedCAl9-9,CA242byECLIA(E1ectro.chemiluminescenceImmunoassay)inperipheralblood.Thedatawereanalysedwi
5、thZtestandFisher,sexacttest.Results:TherateofK-rasgene12pointm似ioninpancreaticcarcinomagroupwas75%(9/12),innon-pancreaticc哪lnomamm撕onmgrouptheratewas5.88%(1/17);ThesensitivityandspecificityofK-rasgenediagnosisofpancreaticcarcinomawere75.00%and94.12%;combiningCAl9-9谢tllK.raSgenemutat
6、ioninparallelexperimentsofpancreaticcancerCan1mproVethediagnosticsensitivityto83.33%.thereisnocorrelationbetweenCAl9-9、CA242、k-rasgenemutationandthepatient’ssex,age,tumorsize,tumorlocationand删stage·(P>aJldK-ras0.05).Conclusions:(1)ThediagnosticspecificityofjointdetectionofCAl9-9dete
7、ctionofgenemutationinperipheralbloodofpatientsishigherthanthatofsingleofk.ras2encmutationinpancreaticcarcinoma,andwhichisconducivetothediagnoslspancreaticcarcinoma.(2)ThereisnocorrelationbetweenCA19-9、CA242、k-rasgenemutationandthepatient·ssex,age,tumorsize,turnorlocationandTNMstages
8、inpancrcaticcarcino
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