赶黄草提取物对大鼠胆汁淤积性肝纤维化tgf-βsmad途径的影响

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1、西南医科大学硕士学位论文InfluenceofTheExtractsfromPenthorumchinesePurshOnTGF一13／SmadSignalingPathwaysinCholestasisLiverFibrosisRatsAbstract：ObjectivesToinvestigatetheinfluenceoftheextractsfromPenthorumchinesePurshonpathologicalchangesandtherapeuticbenefitsincholestasisliverfibrosisratsthroughTGF—p／Smadsig

2、nalpathway．Methods：Cholestasisliverfibrosismodelwassetupbycommonbileductligationofbothendsandthenamiddlecut—offinmaleSDrats．Total40maleSDratswithsuccessfulmodelingwererandomlydividedintofourgroups：commonbileductligationgroup，highandlowdose(49／kg‘1一d1，29／kg-1·d‘1)oftheextractsfromPenthorumchine

3、sePurshgroupgroup，ursodeoxycholicacidgroup(30mg／kg)with10ratsineachgroup．Another10maleSDratswereservedascontrolgrouprandomly．Medicineswerealltakenbygavage，normalandcommonbileductligationgroupratsweregivenequaldoseofnormalsaline．111eratswereanaesthetizedattheendof2ndand4thweekafteroperationtota

4、kethebloodfromheartfordetectingnotonlytheserumlevelof(hyaluronicacid，HA)，(1aminin，LN)，(collagentypeIV，CIV)，butalsoliverfunctionindexes(ALT、AST、ALB、DBIL、TBIL)，meanwhile，partialhepatictissuesofall5groupsratsweretakenforbiopsy．Ifthenudemicediedintheexperimentalprocess，Anautopsyshouldbedoneintime．

5、Allthehepatictissueswerefixedin10％neutral3万方数据西南医科大学硕士学位论文formalin，embeddedinparaffinandstainedwithHEtoobservethepathologicalchangesofhepatictissueandthedegreeofhepaticfibrosis，besidesthedyeingpartsandstainingintensityofTGF一131(transforminggrowthfactor91，TGF-p1)，Smad3，Smad7and伐-SMAweredetermin

6、edbyimmunohistochemistryEnvisionmethod．Results：@SerumLevelofHA，LN，pclII，clVamongursodeoxycholicacidgroupandbothhighandlowdoseoftheextractsfromPenthorumchinesePurshgroupwereallinferiortothoseofcommonbileductligationgroupon14thand28thdayinSDrats，(P<0．05)．@Hepaticfunctionindexes(ALT、AST、ALB、DBIL、

7、TBIL)amongursodeoxycholicacidgroupandbothhighandlowdoseoftheextractsfromPenthorumchinesePurshgroupwereallinferiortothoseofcommonbileductligationgroupon14thand28thdayinSDrats(P<0．05)．@TheexpressionlevelsofTGF-131，Smad3andOc-SMAamongursod