应用mtdna cytb基因全序列分析白唇鹿和五个马鹿亚种的遗传多样性与系统进化

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1、唇鹿的遗传距离为0．049，两者与水鹿的遗传距离无较大差异(0．062^--0．064)，结合系统树说明梅花鹿和白唇鹿几乎同时从水鹿中分化出来。中国马鹿有两个母系起源，白唇鹿有一个母系起源。关键词：白唇鹿；马鹿；线粒体细胞色素b；遗传多样性；系统进化SummaryThistestthewhite-lippeddeer,Gansuwapiti，Qinghaiwapiti，Tianshanwapiti，DongbeiwapitiandTarimwapitiforthestudy,basedonmitochondrialDN

2、ACytBcompletesequence，thecombinationofbioinformaticsmethodstodetectthewhite—lippeddeerandfivesubspeciesofwapitithecytochromeBgenesequencepolymorphism，InordertoprovidescientificmaterialsfortheprotectionandutilizingofChineseCervianebreedsresource．Wesequencedthemit

3、ochondrialDNACytbfor51deersfromwhite-lippeddeerandfivesubspeciesofwapiti．ThealignmentWaScarriedoutbyclustalX．Phylogeneticanalysis，haplotypesandnucleotidediversitywereaccomplishedbyDnasp4．10．MEGA4．0wasusedforconstructingN-JtreeandMEtree．NETWORKWasusedforphylogene

4、ticnetworkconstructions．AccordingtotheGenbankreferencesequence(AJ000022)，therewere28polymorphiesitesin51sequencesof1140bplong，including10locationsoftransitionsand2transversion．17haplotypeswerediscoveredin51sequences．Theproportionis33．3％．Thehighesthaplotypepropor

5、tionis100％inTianshanwapitiandthelowestis0inQinghaiwapiti．GansuwapitiandQinghaiwapitiwerethesameDNAtype．Geneticdiversityofthewhite-lippeddeer,TianshanwapitiandTarimwapitiwereveryrich；Gansuwapiti，Qinghaiwapiti，Dongbeiwapitiwereverypoor．TheanalysisofNJtreeandMEtree

6、showthatthreeobviousbranchesweretakenonwhite—lippeddeerandfivesubspeciesofwapitiinChinese．41sequencesweredownloadedfromGenbank．Including4ofAfricaRedDeer(accessnumber：AY070222、AYl18198、AY244489)，2ofEastAsiaRedDeer(accessnumber：AY070224、AY2444907)，10ofWestEuropean

7、RedDeer(accessnumber：AY070226、AY070221、AY044859、AY244491、AF489281、AY044858、AY044860、AB021099、AY070226、AYl48966)．3ofMiddleEastRedDeer(accessnumber：AYl18199、AF489280)，4ofNorthAsia／America(accessnumber：AF423198、AB021096、AY044862、AF423199)，50fSikaDeer(accessnumber：A

8、B021092～AB021094、AY035876、AB021095)，3ofSouthAsiaRedDeer(accessnumber：AY035875、AY070223、AY044861)．3ofSambar(accessnumber：AY035874、AF423200、AF423201)，7ofBalkanRedDeer(a