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1、HEREDITAS(Beijing)2008年9月,30(9):1187―1194ISSN0253-9772www.chinagene.cn研究报告DOI:10.3724/SP.J.1005.2008.01187中国部分家养山羊mtDNAD环区遗传多样性与进化112222134郝荣超,昝林森,刘丑生,王志刚,张桂香,韩旭,郝怀志,王静,杜小勇1.西北农林科技大学动物科技学院,杨凌712100;2.全国畜牧兽医总站畜禽牧草种质资源保存利用中心,北京100193;3.甘肃农业大学动物科学技术学院,兰州730070;4.华中农业大学动物科技学院,武汉
2、430070摘要:对我国10个家养山羊品种140只个体的mtDNAD-loop区进行测序分析,测得结果:整个D-loop区为1211~1213bp,共检测到84种单倍型,171个多态位点。其中核苷酸多样性(Nucleotidediversity,Pi)为:0.02063±0.00225,单倍型多样性(Haplotypegenediversity,Hd)为:0.988±0.003,平均核苷酸差异数(Averagenumberofnucleotidedifferences)k为:24.896。表明我国家养山羊品种遗传多样性丰富。通过构建NJ网络进化
3、树,得出中国家养山羊主要分为两大支系,并且其中一支与角骨羊(Capraaegagrus)聚在一起,而旋角野山羊(Caprafal-coneri)单独聚为一支,说明角骨羊对中国家养山羊贡献较大。关键词:山羊;mtDNAD-loop;遗传多样性;起源进化GeneticdiversityandoriginofmitochondrialDNAD-loopregionforsomedomesticgoatbreedsofChina1122HAORong-Chao,ZANLin-Sen,LIUChou-Sheng,WANGZhi-Gang,22134ZHA
4、NGGui-Xiang,HANXu,HAOHuai-Zhi,WANGJing,DUXiao-Yong1.CollegeofAnimalScienceandTechnology,NorthwestA&FUniversity,Yangling712100,China;2.NationalCenterforPreservationandUtilizationofGeneticResourcesofDomesticAnimalsandForages,NationalAnimalHusbandry&VeterinaryService,Beijing100
5、193,China;3.AnimalscienceandtechnologyyFacultofGansuAgriculturalUniversity,Lanzhou730070,China;4.AnimalscienceandtechnologyyFacultofHuazhongAgriculturalUniversity,Wuhan430070,ChinaAbstract:ThecompletesequencesofmitochondrialDNAD-loopof140individualsin10Chinesegoat(Caprahiruc
6、)breedswereanalyzedbyDNAsequencingtechnology.TheresultsshowedthatthelengthofmtDNAD-loopinChinesegoatswas1211−1213bp.Therewere84differenthaplotypesand171polymorphicsites.Themeannucleotidediversity(Pi)andhaplotypediversity(Hd)were0.02063±0.00225and0.988±0.003,respectively,andt
7、heaveragenumberofnu-cleotidedifferences(k)was24.896.TheresultsshowedanabundantgeneticdiversityindomesticgoatsofChina.TheNJtreeindicatedthatthereweretwomainbranchesinChinesedomesticgoats,thereinto,onebranchwasclusteredwithCapraaegagrus,andCaprafalconeriwasclusteredalone,which
8、indicatedthatCapraaegagrushadmorecontributiontoChinesedomesticgoatbreeds.收稿
