应用微卫星DNA分析甘肃马鹿遗传多样性及其与青海马鹿的亲缘关系

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1、甘肃农业大学硕士学位论文应用微卫星DNA分析甘肃马鹿遗传多样性及其与青海马鹿的亲缘关系姓名：雷天云申请学位级别：硕士专业：特种经济动物饲养指导教师：滚双宝20090601JISummarY170bloodsamples(Gansureddeer,Qillgllaireddeer,TianQingF1reddeer,TianQingF2reddeerandTarimGanF1reddeer)wereamplifiedanddetected，usingmicrosatelliteDNAmarkers．Anal

2、yzedallelessizeandfrequencyofeachgeneloci；conductedgeneticheterozygosis(He)，polymorphisminformationcontent(PIC)，Nei’Sgeneticdistance(DA)andNei’sStandardGeneticDistance(Ds)；dendrogramwasconstructedusingtheneighbor-joiningandUPGMAmethods；analyzed8locusgenef

3、requencyprincipalconstituentforthe5reddeerpopulations．StudiedgeneticdiversityofGansureddeerandgeneticrelationshipbetweenQm鲥reddeer．Theresultsshowedthat：10micmsatelliteslociwerechoosedtoamplify170bloodsamples，8ofthem(BM203，BM888，BMSl248，TGLA226，BMl818，BM36

4、28，BMCl009andBMl225)wereamplifiedsuccessfully；2ofthem(BM2320andMAF70)wereamplifiedunsuccessfully．Using8microsatelliteslocitodetectgenomeDNAof170bloodsamples，got72alleles，theaveragenumberis9ineachloci．BM888andBMCl009havethehi．曲estnumber(12)；TGLA226hasthelo

5、westnumber(5)．Theaverageallelefrequencyofthe8microsatelliteslocidisU'ibutionissimilarbetweenGansureddeerandQinghaireddeer,7ofthemhavethesamepreponderantalleles．The8microsatelliteslocishowedhi曲PIevalueandpolyrnorphismofGansureddeer,themeanexpectationhetero

6、zygosityWas0．7948；themeancontentofpolymorphisminformationwas0．7566．PhylogeneticanalysisshowedthatNei’SgeneticdistancefDA)valuesandNei’Sstandardgeneticdistance(Ds)valuesbetweenGansureddeerandQinghaireddeerbothweresmaller,i．e．0．0464and0．0227，respectively．Ph

7、ylogenetictreeillustratedthesameconclusion．Principalcomponentanalyses(PCA)indicatedthatGansBreddeerandQinghaireddeerpopulationsaleseparatedfromtheother3reddeerpopulations．TheGansureddeerpopulationshowedhJi业expectationheterozygosityandpolymorphisminformati

8、on,showingrichgeneticdiversity．The8microsatelliteslocicouldbeusedasgeneticIIdiversityanalysisofreddeer．GeneticDifferentiationbasedonmolecularlevelbetweenGansureddeerandQinghaireddeerareinconspicuousness．Inthisstudy，