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1、JournalofForestryResearch,12(1):55-58(2001)55StatusofmicrosatellitesasgeneticmarkersincervidsXUYan-chun,PANZi-chen,XUZhi-ru,YANGShu-hui,JINYu,BAISu-ying(NortheastForestryUniversity,Harbin150040,P.R.China)Abstract:Microsatellitelocidistributingongenomerandomlyacta
2、seffectivegeneticmarkers.Todate,about200microsatellitelociwerefoundincervidsbytransferringmicrosatellitePCRprimersderivedinbovine,ovinetocervids,aswellasafewlocideriveddirectlyfromdeermicrosatellitelibrary.Theselocihavebeenusedinparent-agedetermination,geneticdiv
3、ersityandpopulationstructure,populationintrogression,asgeneticmarkergesta-tionlengthandwinteringsurvivaletal.However,microsatellitelocipresentlyfoundareuntouchabletothedemandofapplication.Futureworkshouldinclude:1)isolatingalargenumberofcervinemicrosatelliteloci,
4、2)constructinggeneticandphysicalmapsofmicrosatelliteloci.Sothatmicrosateliteshaveastrongbaseforadvancedapplica-tionsindeer.Keywords:Deer;Cervids;Microsatellite;GeneticmarkerCLCnumber:Q753;Q959.5Documentcode:AArticleID:1007-662X(2001)01-0055-04sensitivetostudytheh
5、eterozygoteandhomozygote.Characteristicsandsignificanceofmicrosat-TheallelesalsopasstolatergenerationsaccordingelliteDNA1toMendelianlaws.MicrosatellitDNA,therefore,be-comesapowerfulgeneticmarkerandwillbewidelyMicrosatelliteDNAisDNAsequencestandemlyused.repeatingb
6、ythe2-8bpmotifsamongeukaryoticge-nome.BecauseoftheirsimplicityofrepeatmotifandMethodsofobtainingmicrosatellitelociinpatterns,theyarealsocalledShortTandemRepeatsdeer(STRs),SimpleSequenceRepeats(SSRs)orSimpleSequenceLengthPolymorphism(SSLPs)(ChinTherearetwowaystoob
7、tainmicrosatelliteloci1996).TheAbundanceofmicrosatelliteDNAisso(Hammondetal.1998,DeWoodyetal.1995;Engellargeontheeukaryoticgenomethatamicrosatelliteetal.1996;Menotti-Raymondetal.1999):bylocuscanbefoundevery10-50kb(Tautz1989).screeningamicrosatelliteenrichedgeneli
8、brary,andPeoplevaluethemicrosatellitelociduetoitshightransferringmicrosatellitelociderivedincloselyrelat-polymorphismoffragmentlengthcreatedbyitsvaria-edspecie
