杂色鲍微卫星标记的开发及其遗传模式分析

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1、杂色鲍微卫星标记的开发及其遗传模式分析AbstractIt’SwellknownthatHaliotisdiversicolo，．isakindofcommercialabalonefarmedalongthesoutheastcoastofChina,nevertheless，thegermplasmofwhichhasbeenlatelydegenerateandvulnerabletoadversitythankstolongtermartificialinbreeding。Therefore，itseenl

2、simperativetoameliorategeneticbreedingwithmorepowerfulapproach．NodomesticstudyaboutgeneticdiversityofH．diversicolorbymeansofmicrosatellitehaseverbeenreportedthusfar．ThegoalofthisresearchwastoimplorethegeneticdiversityofH．diversicolorinmolecularlevelandlaterser

3、vesasgroundworkforgermplasmimprovement．LaboratoryfocusedonscreeningmicrosatelliteprimersandanalyzinggeneticstructureamongHdiversicolorpopulationsinordertocapturesomeSSRprimersaswellastheoptimumamplificationconditionthatsuppliesfeasibleSSRmarkersandreferencefor

4、furthergeneticbreedingofH．diversicolor．1．PrimerScreening：themixedDNAtemplateof衄diversicolorWasemployedtoscreen83paksofSSRprimersandoptimizetheconditionofreaction．Asaresult，80pairsofutilizableSSRmarkershadcomeavailableforuse．Threepopulationswith30abaloneindivid

5、ualseachgatheredfromSanya,BeihaiandLinggaowereamplifiedundertheoptimalreactionconditionof80pairsofSSRmarkersjusttoyield66pairsofvalidmicrosatellite10ci．2．Analysisonpopulationgeneticstructure：111eaverage嫱ofthree豆diversicolorpopulationsofvariousgeographicalbackg

6、roundsWas0．1076withintermediategeneticdifferentiation．TheamountofallelesfromSanya,BeihaiandLinggaopopulationswere3．6，3．7and3．6respectively,accompaniedbyexpectedheterozygosity(HE)0．45614，0．48423and0．49343aswellasobservedheterozygosity(HO)0．39647，0．40000and0．404

7、04．ThedatamentionedaboveWasmeaningfultomonitoringgeneticdiversityofH．diversicolor,meaningthatall3populationswerewelloffforthetimebeingand，moreover，appropriateSSRlociforgeneticdiversityresearchhadbeendetectedbyvirtueofcontrastiveanalysis．3．Patternsanalysisoffam

8、ilyinheritance：2familiesofdifferentyearsinthesamefarmwereselectedtoscreen80utilizablemicrosatellitelociviaparentstoattain16excellentlyamplifiedloci．Inanalysisofgeneticpatternsoftwo