口服精氨酸促进长骨线性生长的作用及机制的分析

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1、复旦人学硕1学位论文StudyontheeffectandmechanismoforalarginineinimProvingIineargrowthoflongbonesAbstractObjective:(1)ToinvestigateandevaluatetheeffeetoforalL一arginineonimProvingheightgrowthinehildrenwithshortstature.(2)TOinvestigatetheeffeetoforalL一arginineonlineargrowthoflongbonesandexPloret

2、heaetionmeehanism，focusingonthegeneexPressionsrelatedtothehyPothalarnus一Pituitarygrowthaxisandnitrieoxide(NO)一eyelieguanosinemonoPhosPhate(eGMP)PathwaythroughtheexPerieneeinrats.(3)Toobservedtheeffeetoforalarginineonhistologicalstrueturesandultrastrueturesoftherat‘5vitalorgansandeva

3、luatethesaftyofargininefortfCatlneflt.Methods:(l)Incliniealstud又45ehildrenwithshortstatureweregivenoralL一arginine.Beforeandaftertreatment，theheight·bonewidth(BW)bonemineralcontent(BMC)andbonemineraldensity(BMD)weremeasured.ThedatawereanalysedbyPaired一samPles1test.(2)Theratswererand

4、omlydividedintothecontrolgrouPandtheinterventiongrouP.IntheeontrolgrouP:PhysiologicalsalinewasaPPliedforgastriePerfusion，totallyfor4weeks.IntheinterventiongrouP:L一argininewasaPPliedforgastriePerfusion，totallyfor4weeks.Bonehistomo印hometryanalysiswasusedtomeasuregro叭hPlatewidth，minera

5、lapPositionrateofthetibiaandtrabeeularbonevolumefraetion，osteoblastsurfaee，osteoclastsurfaeeofthefemur.Serumgro认thhormone(GH)concentrationwasdeterminedbyradioimmunoassay.Realtime一PCRwasusedtomeasurethegeneexPressionsofneuronalnitricoxidesynthase(nNOS)，solubleguanylyleyclaseal(sGCal)

6、，solubleguanylyleyelase(sGCpl)，cGMP一dePendentProteinkinasell(PKGll)，growthhormone一releasinghormone(GHRll)，somatostatin(55)inhyPothalamusandGHinPituitary.认七sternblotwasusedtodeteettheProteinleversofnNOS，sGCalandsGCplinhyPothalamus.Thedatawereanalysedby1test.(3)Theratswererandomlydivi

7、dedintotheeontrolgrouPandtheintenZentiongrouP.IntheeontrolgrouP:PhysiologiealsalinewasaPPliedforgastriePerfusion，totallyfor12weeks.IntheinterventiongrouP:L一argininewasapPliedforgastriePerfusion，totallyfor12weeks.复日_人学硕l学位论文A丘ertreatment，thehistologiealstructuresandultrastrueturesoft

8、heeerebralcortex、hy