b细胞刺激因子受体baff一r的基因克隆 表达与功能分析

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1、优秀毕业论文Abstr8CtAbstraCtBI娜Phocytestimulator(BLyS)eanregulateBeellsuLrvival，Proliferation，differentiationandm的明拓on.ExeessiveexPressionofBLyScanstimulateBeelltoproliferatemaiignantiywhichresultsintheoecu“enceofal滋oilnlnuJ苗tydiseases.BAFF一R15theSI玲cifierecePto

2、rofBLySwhiehPlaysan而Portantroleinthesurvivalandmat扭ationofBeell.FirSUy，也eex忱鱿ellulardomainofBAFF一Rinieracts诚thBLyS，山entheeomPlexwouldbeassociatedwithinl叭班ellularTRAF3，助dfi耐lyaetivatenon-canonicalNF破Bsignaltr出”duetionPathways.ExtemalBLySreeePtorseanremoveth

3、eexeessiveBLysinvivoaiso.Nowthe丘metionandsignaitransduetionpatilwayofBAFF一Rbeco们。eahotsPotforStUdy‘TbeftmetionofBAFF一R~stU山edfrom幻刀0aspectsasfonows:1.eDNAencodingtheextracellulardomainofBAFF-R、vasgained，thenexPressedby仙iongenewithGST.A刀dtheengineeredstr由na

4、ndfermentationeonditions认吧reoPtlmiZed，subsequentlyWestemBI。币ngwasusedtoidentifytheexpectedProteln.BAFF一Rwasp而五edbyGSTaffinityc加泊matograPhy，theP硕tyofBAFF一wasover85%.L韶Uy，BAFF一Rcanres恤血theProliferationofRA一05eells，whicheanbest访lulatedbyBLysand幻。eas切限dbyMTT.2

5、.cDNAencodingtheintercellulardomainofBAFF一R皿dTRAF3一C、veregaj血ed，thenthemoleCularbindingdomainoftheirinteractionwasStUdiedbytheyeast幻刃o.hybrid.Firstiy，theintercellulardomainofBAFF一Rwasir‘ertedinBDplasmid，TRAF3一CinADplasmid，叨d止enthese勺刃0plas而dswereeotr出招form

6、edtoY190.There，ntIndicatesthattheystrongly油峨‘t.Seeondly，elevendeletionmultantsofTRAF3wereeons廿uetedbydeletingthea“unoaCidsresiduesinNorCte几ninusofTRAF3respectively.T苗rdly，them叮ta叮tsandinierceUulardomainofBAFF一Rwerecotransformedtoyeast.Theresuhindicatesthat

7、the382一00aaeoiled一eoil，428一63aainmiddle，543一560aaofTRAF3areeritiealmoleeular精品参考文献资料优秀毕业论文Abstraetbindingdom滋此fortheinteractionbet认旧enintercellularBAFF一RandTR户f3.TheextracellularregionofBAFF一RProteinProbablycanbeusedforcliinctreatment，曲d也einten比tiondom和i朴o

8、ftheintereellularBAFF一RandTRAF315thebasisfortheinvestigationoftheliltercellularsignal加劝sduction.Keywords:BAFF一R;exPressionofProteins;TR八f3:themolecularbindingdomain111厂厂精品参考文献资料优秀毕业论文目录................目录第1章文献