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1、·ABSTRACTABSTRACTAegilopsbiuncialis(2n=4x=28，UbUbMbMb)isawildtetraploidlineofAegilopsgenusAegilopsumbellulataspecies.Asathirdgeneresourceofwheat,Ae.biuncialiscarrymanyoutstandingagronomicalfeatures,forexample,droughttolerance,salttolerance,insectandpestresistance,diseaseresista
2、nce,inadditionto,italsohasspecialhighmolecularweightgluteninsubunits(HMW-GSs).Therefore,Itcanbeusedasanimportantandpotentialsourceforwheatimprovement.Untilnow,onlyMolnár-LángMandhisteamhavecarriedoutsomeworkofAe.biuncialisinrecentyears.Soweneedtofurthertransfertheusefulgenesfro
3、mAe.biuncialisintowheatviascreeningitsadditionlines,substitutionlines,ortranslocationlines.Inthestudy,Ae.biuncialiswascrossedtowheatlineCN19toproduceF1hybridplants.andthenthehybridplantsweretreatedwithcolchicine,weobtainedwheat-Ae.Biuncialisamphiploid(AABBDDUUMM,2n=10x=70).Afte
4、rpreliminaryidentificationofthedescendantsofthehybridizationbetweenCN19andwheat-Ae.biuncialisamphiploid,weobtainageneticallystableline12-5-2,whichcontainsspecialhighmolecularweightgluteninsubunitsfromAe.biuncialis.Chromosomenumbersinroot-tipcellsof12-5-2were2n=44,anditwasimmune
5、tobothstriperustandpowderymildew.Itwasconfirmedthat12-5-2wasa1Ubdisomicadditionline(ADL1Ub)byfluorescenceinsituhybridizationandC-banding.seedproteinSDS-PAGEanalysisshowedthat12-5-2notonlyownedallhighmolecularweightgluteninsubunits(7+8and2+12)fromitsparentCN19,butalsohad2newsubu
6、nits,whichwerenamedUxandUy.22PLUGprimersfromhomologousgrouponeand26PLUGprimersthatweredesignedwereusedtoamplifyCS,Ae.biuncialis,CN19,12-5-2,weobtained5pairs1Ub-specificPLUGprimers,TNAC1021,TNAC1041,TNAC1071,TNAC1-01,andTNAC1-04.Whatismore,wedetermined
theflourqualityparameterso
7、f12-5-2anditsparentCN19,itshowedthattheprotein
content,wetglutencontent,grainhardnessandZelenysedimentationvalueof12-5-2
wereapparentlyhigherthanthoseofitsparentCN19.Insummary,weusethemethodsofbiochemistry,morphologycytology,and
molecularbiologytoidentitytheBC3F2descendantsofhy
8、bridization12-5-2.thenew
ADL1Ub12-5-2couldbeathirdgene
