d-半乳糖对成年小鼠海马神经元p—erk12

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1、D-半乳糖对成年小鼠海马神经元p—ERK1／2表达和学习记忆能力的影响曹雪姣1，耿成燕2，董伟2，张翠丽1，刘爽2，李秋娟2（1.大连医科大学生物技术专业，大连116044；2.大连医科大学预防医学实验室，大连116044）【摘要】目的研究D-半乳糖对成年小鼠海马神经元p—ERK1／2表达和学习记忆能力的影响。方法20只健康成年昆明种小鼠，雌雄各半，分为2组。颈背部皮下注射D-半乳糖注射液，每天125mg/kgbw，连续35d，建立小鼠衰老模型。对照组注射生理盐水。其间观察小鼠一般状况，实验的最后1周分别进行跳台实验

2、、Morris水迷宫实验检测小鼠的学习记忆能力；实验结束后处死动物，提取海马组织总蛋白，免疫印迹检测p—ERK1／2表达量。结果与对照组比较，D-半乳糖组小鼠跳台试验潜伏时间减少和电击次数增加（P<0.01）；Morris水迷宫实验航行逃避潜伏期明显大于对照组小鼠（P<0.01）；D-半乳糖组p-ERK1/2表达量显著低于正常对照组（P<0.01），且雄性比雌性效果显著。结论D-半乳糖可能通过抑制成年小鼠海马神经元p—ERK1／2表达而损害其学习记忆能力。【关键词】D-半乳糖；衰老；学习记忆功能；p-ERK1/2【中

3、图分类号】R332【文献标识码】A【文章编号】YX2013366TheeffectsofD-galactoseontheexpressionofp-ERK1/2inhippocampusofmiceandonlearningandmemoryabilityCAOXue-jiao，GENGCheng-yan，DONGWei，ZHANGCui-li，LIIUShuang，LIQiu-juan(1.DepartmentofBiotechnology,DalianMedicalUniversity,Dalian116044,

4、China;2.DepartmentofPreventivemedicinelaboratory,DalianMedicalUniversity,Dalian116044,China)[Abstract]ObjectiveTostudytheeffectsofD-galactoseontheexpressionofp-ERK1/2inhippocampusofmiceandonlearningandmemoryabilityMethodsTwentyKunmingmice,halfmaleandhalffemale,

5、weredividedintotwogroups.Toestablishagingmodelofmice,D-galactosewasinjecteddailyinthesubcutaneousfor35daysinthedosageof125mg/kg.Thecontrolgroupwasgivensaline.Inthemodelingperiod,thegeneralconditionofmicewasobserved.Step-downandmorriswatermazewerecarriedouttotes

6、tthelearningandmemoryabilityatthelastoneweek.Aftertheexperiment,theanimalsweresacrificedtoextracthippocampaltissue.Theexpressionofp-ERK1/2inhippocampalofmicewasdetectedbywesternblot.ResultsStep-downtestshowedthatcomparedwiththecontrolgroup,thelatencyinthemodelg

7、roupdecreasesignificantly(P<0.01)andthenumberofelectricshockinthemodelgroupincreasesignificantly(P<0.01).Morriswatermazeshowedthatthelatencyofescapeinthemodelgroupwerelongerthanthecontrolgroup(P<0.01).Westernblottingresultsshowedthattheexpressionofp-ERK1/2signi

8、ficantlydecreasedinthemodelgroupandtheeffectismorepronouncedinmalemice.ConclusionD-galactosemayimpairlearningandmemoryabilitybyinhibitingtheexpressionofp-ERK1/2inhippocampal