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1、·精元康胶囊对骨髓抑制小鼠造血功能及凋亡相关蛋白的影响达、下调Bax蛋白的表达，优于利可君组和模型组（P<0.05）；精元康胶囊低剂量组在上调骨髓有核细胞Bcl-2蛋白表达、下调Bax蛋白表达方面也有一定效果。结论：精元康胶囊能有效地改善CTX致骨髓抑制小鼠的骨髓功能，其作用机制可能为：①精元康胶囊具有显著提高外周血白细胞、红细胞、血红蛋白和血小板的作用，尤其在提高外周血白细胞和血小板方面作用明显；②精元康胶囊具有显著提升骨髓有核细胞数的作用；③精元康胶囊在上调Bcl-2蛋白表达的同时，下调Bax蛋白表达，使Bcl-2/Bax比值增大，防止骨髓造血细胞凋亡。本实验
2、结果表明，精元康胶囊具有抗骨髓细胞凋亡作用，这可能是在骨髓抑制状态下，精元康胶囊促进骨髓细胞增殖和骨髓造血功能恢复的机制之一。关键词：小鼠；骨髓抑制；环磷酰胺；精元康胶囊；利可君片；骨髓有核细胞数；凋亡相关蛋白···-2-···AbstractEffectofJingyuankangCapsuleonHematopoieticFunctionandApoptosis-associatedProteinsofMyelosuppressedMiceWangHongling(InternalMedicineofTCM)Directedby:ProfessorJiangS
3、hiqingAbstractObjective:ToobservetheeffectofJingyuankangCapsuleonleucocyte,erythrocyte,hemoglobin,platelet,bonemarrowcellandapoptosis-associatedproteinsonmyelosuppresseofmiceinducedbycyclophosphamide(CTX),andtofindoutthemechanismofJingyuankangCapsuleinamelioratingthehematopoieticfunct
4、ionofmyelosuppressedmice,whichwouldprovidetheexperimentalevidenceforclinicapplication.Methods:Select144KMmice(male,6~8weeksage,18~22gweight),whichwererandomlydividedinto6groups(normalgroup,modelgroup,Likejuntabletgroup,JingyuankangCapsulehigh-dosegroup,JingyuankangCapsulemedium-dosegr
5、oup,JingyuankangCapsulelow-dosegroup),24miceineachgroup.Between1~3rddayeverymorning,eachgroupwasadministratedbygastricperfusionofCTXinjection100mg/kg(normalgroupwasinjected0.9%NSbyabdominalcavity.);Between1~9thdayeveryafternoon,normalgroupandmodelgroupwereadministratedbygastricperfusi
6、onof0.5%CMC-Na,LikejunTabletgroupwastreatedwithLikejunTablet32mg/20ml/kg,JingyuankangCapsulehigh-dosegroupwithJingyuankangCapsule4g/20ml/kg,JingyuankangCapsulemedium-dosegroupwithJingyuankangCapsule3g/20ml/kg,JingyuankangCapsulelow-dosegroupwithJingyuankangCapsule2g/20ml/kg.Between1~1
7、0thdayeverymorning,randomlyselect8micefromeachgroup,cuttheirtailsinturntoobtain20μlbloodeach,dripintotesttubesofEDTA-K2dilutiontobeusedforinspectinghemogramandprintingdatabyautomaticbloodcellanalysator.Onthe5th,7th,10thday,select8micerandomlyfromeachgroupforexecution,takeoutthebonemar
8、rowce
