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1、正交设计法优选六月青多糖胶囊提取工艺葛文漪，黄建春，陈兆霓，焦阳，张士军，黄仁彬*(广西医科大学药理学教研室，南宁530021)[摘要]目的：优选六月青多糖胶囊提取工艺的最佳条件。方法：选用L9(34)正交试验设计方法，分别对水煎煮及醇沉两个步骤进行优选，以多糖含量为水煎煮工艺评价指标；采用苯酚-浓硫酸法测定总多糖含量，以多糖含量和收率为醇沉工艺综合评价指标，确定最佳提取工艺参数。结果：六月青多糖胶囊最佳提取工艺为生药材加10倍量水，每次煎煮2h，煎煮3次。水提液过滤、合并、浓缩至含生药0.5g·mL-1，7倍量95%乙醇沉淀12h，沉淀1次。结论：优选出的工艺条件,经验证其结果稳定、合理
2、，可作为六月青多糖胶囊的最佳提取工艺。[关键词]六月青胶囊；正交试验；提取工艺；煎煮法；醇沉法OptimizationofExtractionTechnologyforLiuyueqingPolysaccharidesCapsulebyOrthogonalDesignGEWen-yi,HUANGJian-chun,CHENZhao-ni,JIAOYang,ZHANGShi-jun,,HUANGRen-bin(DepartmentofPharmacology,GuangxiMedicalUniversity,Nanning530021,China)[Abstract]Objective:To
3、optimizethetechnologyofextractionforLiuyueqingpolysaccharidescapsules.Method:ThetechnicalconditionsforthewaterdecoctionandthealcoholprecipitationwereoptimizedrespectivelybyorthogonalexperimentdesignL9(34).Thecontentofpolysaccharideswasusedastheindexforthewaterdecoction,thecontentofpolysaccharidesa
4、ndextractratewereusedasindexesforalcoholprecipitation.Themethodofphenol-sulfuricacidreactionwasusedindeterminingthecontentofpolysaccharides.Optimalprocesscontrolparameterswere______________________________[收稿日期][基金项目]广西科学研究与技术开发计划项目(桂科攻10124008-6，桂科攻0992003A-2，KFJJ2010-22)[第一作者]葛文漪，硕士研究生，从事药物质量控制和
5、生化药理学研究，Tel:15994443433,E-mail:gewenyi1986@163.com[通讯作者]*黄仁彬，教授，博士研究生导师，从事抗病毒性肝炎药物和生化药理学研究，Tel:0771-5339805,E-mail:huangrenbin518@163.comdetermined.Result:TheoptimalconditionsforLiuyueqingpolysaccharidescapsuleswereasfollows:decoctingthecrudedrugsthreetimeswith10-foldwater,2hourseachtime.Thephysic
6、liquorextractedbywaterwasfiltered,mixedandconcentratedto0.5g·mLl-1(crudedrug),andthenprecipitatedby7-fold95%alcoholonetimefor12hours.Conclusion:Theresultofthisoptimizedextractionprocedureisprovedtobestableandreliable,itcanbeusedastheoptimalextractionprocedureofLiuyueqingpolysaccharidescapsules.[Ke
7、ywords]Liuyueqingpolysaccharidescapsules,orthogonalexperimentdesign,extractiontechnology,decoctionmethod,alcohol-precipitationmethod六月青为广西特色民间草药，系爵床科(Acanthaceae)肖鸡笼属植物肖鸡笼（顶花马兰）Taraphochlamysaffinis(Giff)Bremekhu
