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1、Extractionandseparationskin-whiteningagentfromRhizomaChuanxionganditsapplicationincosmeticsAbstractZHANGZhiping(TraditionalChinesemedicine)Supervisor：HEQiuxing,AssociateprofessorChuanxiongisthedryrootofLigusticumChuanxiongHort,perennialherb,whichhasmanypharmacologicallyactivi
2、tyofbloodcirculation,qiGloomy,chillsandpain.BytracingtheactivityoftyrosinaseinhibitiontofindtheactiveingredientinChuanxiong,andusedincosmetics.DevelopedChuanxiongcreamwithwhiteningeffect,providingexperimentalevidenceandtheoreticalfoundationforthedevelopmentofthemarket.Themain
3、researchresultsareasfollows.1)Theoptimumreactionconditionsofinhibitionontyrosinaseactivity.Arbutinastheresearchobject,bythesinglefactorexperimentmethodtostudythephosphatebuffer,substrateL-tyrosine,tyrosinase,holdingtimeandresponsetimerespectively,studyitsinfluenceoninhibittyr
4、osinaseactivity.TheexperimentalresultsshowthatthepHvalueofPhosphateBufferSolutionwas6.8，thevolumeofL-Tyrosinewas1.0mL,thevolumeoftyrosinasewas1.0mL,timeinwaterbathbeforetyrosinaseaddedwas10minutesandreactingtimewas10minutes.2)ScreeningofwhiteningChinesemedicine.Thewaterandeth
5、anolextractsofRadixOphiopogonis,Radixastragali,ChuanxiongandmotherwortweredetermineditscrudeextractinhibittyrosinaseactivityandremovetheeffectofDPPH,respectively.ScreeningthetraditionalChinesemedicinewithwhiteningandanti-oxidationeffect.TheresultsshowedthatChuanxionghasthestr
6、ongerinhibitionthanothers;Chuanxiongandmotherworthasstrongantioxidantactivity.Therefore,theChuanxiongwasthepotentialdevelopmentmedicineforwhiteningefficacy.3)TheprocessingconditionsofethanolextractofChuanxiongwereoptimized.Ontyrosinaseactivityrateastheindex,theprocessingcondi
7、tionsofethanolextractofIII万方数据Chuanxiongwereoptimizedwithsinglefactorexperimentandorthogonaldesigntest.Theresultsshowedthattheoptimumextractingconditionwasobtainedastheconcentrationofethanolwas70%,timeforrefluxingwas3h,theratioofmaterialandsolventwas1：10andrepeattheprocessfor
8、2times.4)ThewhiteningactiveingredientisolatedfromChuanxiong.Theethan
