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1、Extractionandseparationskin-whiteningagentfromRhizomaChuanxionganditsapplicationincosmeticsAbstractZHANGZhiping(TraditionalChinesemedicine)Supervisor：HEQiuxing,AssociateprofessorChuanxiongisthedryrootofLigusticumChuanxiongHort,perennialherb,whichhasmanypharmacological
2、lyactivityofbloodcirculation,qiGloomy,chillsandpain.BytracingtheactivityoftyrosinaseinhibitiontofindtheactiveingredientinChuanxiong,andusedincosmetics.DevelopedChuanxiongcreamwithwhiteningeffect,providingexperimentalevidenceandtheoreticalfoundationforthedevelopmentoft
3、hemarket.Themainresearchresultsareasfollows.1)Theoptimumreactionconditionsofinhibitionontyrosinaseactivity.Arbutinastheresearchobject,bythesinglefactorexperimentmethodtostudythephosphatebuffer,substrateL-tyrosine,tyrosinase,holdingtimeandresponsetimerespectively,study
4、itsinfluenceoninhibittyrosinaseactivity.TheexperimentalresultsshowthatthepHvalueofPhosphateBufferSolutionwas6.8，thevolumeofL-Tyrosinewas1.0mL,thevolumeoftyrosinasewas1.0mL,timeinwaterbathbeforetyrosinaseaddedwas10minutesandreactingtimewas10minutes.2)Screeningofwhiteni
5、ngChinesemedicine.ThewaterandethanolextractsofRadixOphiopogonis,Radixastragali,ChuanxiongandmotherwortweredetermineditscrudeextractinhibittyrosinaseactivityandremovetheeffectofDPPH,respectively.ScreeningthetraditionalChinesemedicinewithwhiteningandanti-oxidationeffect
6、.TheresultsshowedthatChuanxionghasthestrongerinhibitionthanothers;Chuanxiongandmotherworthasstrongantioxidantactivity.Therefore,theChuanxiongwasthepotentialdevelopmentmedicineforwhiteningefficacy.3)TheprocessingconditionsofethanolextractofChuanxiongwereoptimized.Ontyr
7、osinaseactivityrateastheindex,theprocessingconditionsofethanolextractofIII万方数据Chuanxiongwereoptimizedwithsinglefactorexperimentandorthogonaldesigntest.Theresultsshowedthattheoptimumextractingconditionwasobtainedastheconcentrationofethanolwas70%,timeforrefluxingwas3h,t
8、heratioofmaterialandsolventwas1：10andrepeattheprocessfor2times.4)ThewhiteningactiveingredientisolatedfromChuanxiong.Theethan
