大鼠延髓背角和脊髓背角内pkcγ阳性神经元向中缝隐核的投射论文

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1、大鼠延髓背角和脊髓背角内PKCγ阳性神经元向中缝隐核的投射论文【摘要】目的研究大鼠延髓背角和脊髓背角浅层内蛋白激酶Cγ亚单位(PKCγ)阳性神经元向中缝隐核(NRO)的投射。方法应用荧光金(FG)逆行追踪和PKCγ免疫荧光组织化学染色相结合的双标记方法，观察大鼠延髓背角和脊髓背角内PKCγ阳性神经元向中缝隐核的投射。结果将FG注入NRO，在延髓背角和脊髓背角的Ⅰ～Ⅲ层内可见FG逆标神经元；PKCγ阳性神经元主要分布于延髓背角和脊髓背角的Ⅱ层内侧部及Ⅱ、Ⅲ层交界处，Ⅰ、Ⅲ层内较少；延髓背角Ⅰ层和脊髓背角Ⅰ～Ⅲ层可观察到FG逆标并

2、呈PKCγ阳性的双标神经元。结论延髓背角和脊髓背角浅层向NRO投射的神经元为PKCγ阳性神经元，其在向NRO传递信息的通路中可能发挥重要作用。【关键词】蛋白激酶C中缝隐核延髓背角脊髓背角荧光免疫测定大鼠［ABSTRACT］ObjectiveToinvestigatetheprojectionsofproteinkinaseCγisoform(PKCγ)immunoreactiveneuronsfromthemedullaryandspinaldorsalhornstothenucleusrapheobscurus(NRO)in

3、rat．MethodsThedoublelabelingmethodoffluorogold(FG)retrogradetracingbinedmunofluorescencehistochemicalstaininginaeⅠ—Ⅲofthemedullaryandspinaldorsalhorns.PKCγimmunoreactiveneuronsainlyobservedintheinnerpartoflaminaⅡandtheborderregionbetinaeⅡandⅢ，inaeⅠandⅢ，onlyafemunor

4、eactiveneuronseFG1abeledneuronsinlaminaⅠofthemedullaryandlaminaeⅠ—ⅢofthespinaldorsalhornalsoexhibitedPKCγimmunoreactivity．ConclusionThepresentresultsindicatethatPKCγcontainingneuronsinthemedullaryandspinaldorsalhornsprojecttotheNRO.freelightbeinvolvedinthetransmiss

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