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时间:2018-11-22
《大鼠延髓背角和脊髓背角内pkcγ阳性神经元向中缝隐核的投射论文》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、大鼠延髓背角和脊髓背角内PKCγ阳性神经元向中缝隐核的投射论文【摘要】目的研究大鼠延髓背角和脊髓背角浅层内蛋白激酶Cγ亚单位(PKCγ)阳性神经元向中缝隐核(NRO)的投射。方法应用荧光金(FG)逆行追踪和PKCγ免疫荧光组织化学染色相结合的双标记方法,观察大鼠延髓背角和脊髓背角内PKCγ阳性神经元向中缝隐核的投射。结果将FG注入NRO,在延髓背角和脊髓背角的Ⅰ~Ⅲ层内可见FG逆标神经元;PKCγ阳性神经元主要分布于延髓背角和脊髓背角的Ⅱ层内侧部及Ⅱ、Ⅲ层交界处,Ⅰ、Ⅲ层内较少;延髓背角Ⅰ层和脊髓背角Ⅰ~Ⅲ层可观察到FG逆标并
2、呈PKCγ阳性的双标神经元。结论延髓背角和脊髓背角浅层向NRO投射的神经元为PKCγ阳性神经元,其在向NRO传递信息的通路中可能发挥重要作用。【关键词】蛋白激酶C中缝隐核延髓背角脊髓背角荧光免疫测定大鼠[ABSTRACT]ObjectiveToinvestigatetheprojectionsofproteinkinaseCγisoform(PKCγ)immunoreactiveneuronsfromthemedullaryandspinaldorsalhornstothenucleusrapheobscurus(NRO)in
3、rat.MethodsThedoublelabelingmethodoffluorogold(FG)retrogradetracingbinedmunofluorescencehistochemicalstaininginaeⅠ—Ⅲofthemedullaryandspinaldorsalhorns.PKCγimmunoreactiveneuronsainlyobservedintheinnerpartoflaminaⅡandtheborderregionbetinaeⅡandⅢ,inaeⅠandⅢ,onlyafemunor
4、eactiveneuronseFG1abeledneuronsinlaminaⅠofthemedullaryandlaminaeⅠ—ⅢofthespinaldorsalhornalsoexhibitedPKCγimmunoreactivity.ConclusionThepresentresultsindicatethatPKCγcontainingneuronsinthemedullaryandspinaldorsalhornsprojecttotheNRO.freelightbeinvolvedinthetransmiss
5、ionofnociceptiveinformationfromthemedullaryandspinaldorsalhornstotheNRO.[KEYAI,FIELDSHL.Endogenouspaincontrolsystem:brainstemspinalpathmainratspinalcord[J].BrainResearch,1999,833:7173.[3]MALMBERGAB,CHENC,TONIGAARTINmationinducedupregulationofproteinkinaseCimmunor
6、eactivityinratspinalcordcorrelatesmalikeimmunoreactivityoftrigeminothalamicneuronsinthemedullarydorsalhornoftherat[J].BrainRes,2001,913:159163.[7]吴利平,李辉,李云庆,等.大鼠延髓和脊髓背角内PKCγ阳性神经元向丘脑胶状质的投射[J].解剖学杂志,2002,25(2):101104.[8]NITS,bindingproteinsinneuronsofthemedullaryd
7、orsalhornoftherat[J].Neurosignals,2005,.freeledullarydorsalhorn:anintracellularlabelingstudyintherat[J].JpNeurol,1999,411(3):399402.[10]LIJL,LIYQ,SAKASHIN,etal.ProteinkinaseCgammalikeimmunoreactivityinthesubstantiagelatinosaofthemedullarydorsalhornoftherat[J].Neur
8、osciLett,2001,311:159163.
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