资源描述：
《不同基原秦皮、香豆素单体抗菌作用对比研究论文》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、不同基原秦皮、香豆素单体抗菌作用对比研究论文.freelarinmonomer,5kindsofmonomermixture,knoarinfingerprintregionsampleandunknoentinvitroandmicroamountandquantitativedetectionmethodseasureminimuminhibitoryconcentration(MIC)andminimumbactericidalconcentration(MBC).ResultFourkin
2、dsoforiginsashbarkincludingFraxinusrhynchophyllaHance,FraxinuschinensisRoxb.var.acuminataLingelsh.,Fraxinuschinensis,FraxinusstylosaLingelsh.hadobviousbacteriostasisandbactericidalaction,bacteriostasisintensityorderinataLingelsh.＞Fraxinuschinensis.Fiv
3、ekindsofcoumarinmonomer：fraxetin,aesculetinhadobviousbacteriostasisandbactericidalaction,aesculinandfraxinhadcertainbacteriostasisandbactericidalactiontoStaphylococcusaureus,Staphylococcusepidermidis.Bacteriostasisandbactericidalactionorderethoxyl-8-H
4、ydroxycoumarin.FraxinusrhynchophyllaHanceextracthadobviousbacteriostasisto9kindsofbacterium；monomermixtureandcoumarinfingerprintregionsamplehadobviousbacteriostasistoStaphylococcusaureus,Staphylococcusepidermidis,betastreptococcus,aeruginosusBacillus,
5、Bacilluscoli,Klebsiellapneumoniae,catarrh.UnknoplehadobviousbacteriostasistoStaphylococcusaureusandcertainbacteriostasistoStaphylococcusepidermidis,alphastreptococcus,betastreptococcus,Bacilluscoli,salmonella.ConclusionThechangesofashbarkbacteriostasi
6、sarinponentcontent.Aglyconebacteriostasisofashbarkostpoarinmonomer.Keyarinmonomer；bacteriostasis秦皮为木犀科植物苦枥白蜡树FraxinusrhynchophyllaHance、白蜡树FraxinuschinensisRoxb.、尖叶白蜡树FraxinusszaboanaLingelsh.、宿柱白蜡树FraxinusstylosaLingelsh.的干燥枝皮或干皮,具有清热燥湿、收涩、明目的功能,用于热痢
7、、泄泻、赤白带下、目赤肿痛、目生翳膜等。秦皮主要含有香豆素类化学成分,如：秦皮甲素(aesculin)、秦皮乙素(aesculetion)、秦皮苷(fraxoside)、秦皮素(fraxetol)、6,7-二甲氧基-8-羟基香豆素1-2。本研究对4种基原秦皮提取物、5种香豆素单体、5种单体混合物和以苦枥白蜡树树皮为代表的秦皮提取物、已知香豆素指纹区样品、未知成分指纹区样品进行了体外抑菌试验,旨在阐明其抑菌、杀菌作用的强弱,为客观地评价秦皮质量、指导临床用药提供科学依据。1实验材料1.1药物4种基原
8、秦皮：苦枥白蜡树、尖叶白蜡树、白蜡树、宿柱白蜡树树皮提取物；5种香豆素单体：秦皮甲素、秦皮乙素、秦皮苷、秦皮素、6,7-二甲基-8-羟基香豆素(均为自制,纯度＞98%)；以苦枥白蜡树为代表的秦皮提取物,5种香豆素单体混合物(按苦枥白蜡树提取物中各单体含量：秦皮甲素2.08%、秦皮乙素0.14%、秦皮苷0.84%、秦皮素0.37%、6,7-二甲基-8-羟基香豆素0.03%混合而成)；苦枥白蜡树已知香豆素指纹区样品,未知成分指纹区样品。上述样品以DMSO溶解,实验前配制。1.2试剂及培
