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1、不同基原秦皮、香豆素单体抗菌作用对比研究：刘丽梅,王瑞海,陈琳,杨庆,翁小刚,孙建辉【摘要】目的研究4种基原秦皮提取物、5种香豆素单体、5种单体混合物、苦枥白蜡树树皮已知香豆素指纹区样品、未知成分指纹区样品的体外抑菌作用。方法采用体外抑菌试验微量定量检测方法,测定最小抑菌浓度及最低杀菌浓度。结果4种基原秦皮苦枥白腊树、尖叶白腊树、白腊树、宿柱白腊树树皮均有明显抑菌、杀菌作用,抑菌强度依次为：宿柱白腊树＞苦枥白腊树＞尖叶白腊树＞白腊树。5种香豆素单体秦皮素、秦皮乙素对9种细菌均有明显抑菌、杀菌作用,秦皮甲素、秦皮苷对金黄色葡萄球菌、表皮葡萄球菌有一定抑菌、杀
2、菌作用,抑菌作用依次为：秦皮素＞秦皮乙素＞秦皮甲素＞秦皮苷＞6,7-二甲氧基-8-羟基香豆素。苦枥白腊树树皮提取物对所试9种细菌均有明显抑菌作用；单体混合物、已知香豆素指纹区样品对金黄色葡萄球菌、表皮葡萄球菌、乙型溶血性链球菌、绿脓杆菌、大肠杆菌、肺炎克雷伯氏菌、卡他菌有明显抑菌作用；未知成分指纹区样品对金黄色葡萄球菌有明显抑菌作用,对表皮葡萄球菌、甲型溶血性链球菌、乙型溶血性链球菌、大肠杆菌、沙门氏菌也有一定抑菌作用。结论秦皮抑菌强弱与已知香豆素成分含量高低基本一致。秦皮中苷元的抑菌作用优于苷,5种香豆素类单体中秦皮素、秦皮乙素抑菌作用最强。【关键词】秦
3、皮；香豆素；抑菌作用Abstract：ObjectiveTostudybacteriostasisinvitroof4kindsoforiginsashbarkextract,5kindsofcoumarinmonomer,5kindsofmonomermixture,knoarinfingerprintregionsampleandunknoentinvitroandmicroamountandquantitativedetectionmethodseasureminimuminhibitoryconcentration(MIC)andminimumba
4、ctericidalconcentration(MBC).ResultFourkindsoforiginsashbarkincludingFraxinusrhynchophyllaHance,FraxinuschinensisRoxb.var.acuminataLingelsh.,Fraxinuschinensis,FraxinusstylosaLingelsh.hadobviousbacteriostasisandbactericidalaction,bacteriostasisintensityorderinataLingelsh.＞Fraxinusc
5、hinensis.Fivekindsofcoumarinmonomer：fraxetin,aesculetinhadobviousbacteriostasisandbactericidalaction,aesculinandfraxinhadcertainbacteriostasisandbactericidalactiontoStaphylococcusaureus,Staphylococcusepidermidis.Bacteriostasisandbactericidalactionorderethoxyl-8-Hydroxycoumarin.Fra
6、xinusrhynchophyllaHanceextracthadobviousbacteriostasisto9kindsofbacterium；monomermixtureandcoumarinfingerprintregionsamplehadobviousbacteriostasistoStaphylococcusaureus,Staphylococcusepidermidis,betastreptococcus,aeruginosusBacillus,Bacilluscoli,Klebsiellapneumoniae,catarrh.Unknop
7、lehadobviousbacteriostasistoStaphylococcusaureusandcertainbacteriostasistoStaphylococcusepidermidis,alphastreptococcus,betastreptococcus,Bacilluscoli,salmonella.ConclusionThechangesofashbarkbacteriostasisarinponentcontent.Aglyconebacteriostasisofashbarkostpoarinmonomer.Keyarinmono
8、mer；bacteriostasis秦皮为木犀科植物苦枥白蜡树Fr
