细胞膜钙atp酶在心肌缺血-再灌注和腺苷诱导预适应中的作用

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1、细胞膜钙ATP酶在心肌缺血/再灌注和腺苷诱导预适应中的作用【摘要】目的：探讨在心肌缺血/再灌注和腺苷诱导预适应过程中细胞膜钙泵（PMCA）的可能作用。方法：将培养的乳鼠心肌细胞分为3组：缺血/再灌注组、腺苷预处理组和对照组。各组乳酸脱氢酶（LDH）漏出量以生化法检测（n＝5）；半定量RTPCR检测各组PMCA基因转录水平（n＝3）；PMCA活性以定磷比色法测定（n＝5）。结果：（1）缺血/再灌注组LDH漏出量明显增高(P<0.001)，腺苷预处理组LDH漏出量显著降低(P<0.001)；（2）各组间PMCAmRNA转录水平

2、无显著性差异（P>0.05)；（3）各组间PMCA蛋白活性无显著性差异（P>0.05)。结论：在乳鼠心肌细胞缺血/再灌注过程中PMCA无代偿功能；PMCA不直接参与腺苷诱导预适应的心肌保护作用。【关键词】钙ATP酶钙再灌注损伤药物预处理大鼠SpragueDaicphosphate(32Pcolloid)onthelatentmetastasisduringlungcancerresection.Methods73patientsorandinterstitialadministrationof32Pcolloid,by

3、thesametimespan,58patientsediagnosisundericdistributionofbodysurface32Pcolloidactivity,incidenceofplication,ratesofsupraclaviclelymphnode(SCL)metastasisindifferentpathologicpatterns,andsurvivalratesat1,3,5yearphnodemetastasisandtheincidencesofmajorplicationafteroper

4、ationetastasisinsurgeryplus32Pcolloidgroupergroupphaticmetastasisandlocalrecurrence,andissurelytoprolongthesurvivaltimeofpatients.Keyphaticmetastasis;operativetreatment;radiotherapy;32Pcolloid;phosphorusradioisotopes[CLCnumber]R734.2;R817.541[Documentcode]A[ArticleID]1

5、6716264(2008)04024406ChinaisoneofthecountriesinAsiaokingratesarenooreandmorefrequentasaconsequence.Inordertogivepatientsanymethodshavebeentriedincludingimprovementofsurgicalresection,amorethoroughlyintrapulmonarylymphnodesdissection,andabetterchemotherapyandradiothera

6、py［12］.Interstitialirradiationusing32Pchromicphosphate(32Pcolloid)onthelatentmetastasisduringlungcancerresectionisaneethodandhasneverbeenreported.FromMarch1997toMarch2001,lychoose103patientsorandgavethemtheinterstitialinjectionof32Pcolloidbythesametimespan.ethodof32P

7、colloidadministration32Pcolloideterofcolloidgranule.Theadministratedactivityforpatientsarysolutionshouldbedilutedto10mlalsaline,ixedandaspiratedintoasterilesyringeforuse.Duringoperation,afterresectionofdiseasedlungandvisibleLN,infiltrativeinjectionor,neighbortothepulmo

8、naryhilar,carina,uppermediastinumandthesitessuspectedtohavemetastasis.Intheprocessingoftheprimarysoluti