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1、XRCC2基因多态性与非小细胞肺癌易感性的关系论文王瑞，曾辉，李琰，王娜，张健慧，张金文，王伟，刘俊峰【关键词】非小细胞肺癌；XRCC,2基因；易感性；遗传多态性AssociationbetorphismofXRCC2andsusceptibilityofnonsmallcelllungcancers1DepartmentofThoracicSurgery,FourthAffiliatedHospital,HebeiMedicalUniversity,2LaboratoryofMolecularBiology,HebeiCancerInstitute,Shijiazhu
2、ang050011,China,3NationalCenterforAIDSControlandPrevention,ChineseCenterforDiseaseControlandPrevention,Beijing100050,China,4OperationRoom,.freelentofSurgery,ChenganCountyHospitalofHebeiProvince,Handan056700,China【Abstract】AIM:Toobservetheassociationbetallcelllungcancersandpolymorphismand
3、genefrequencyofXRCC2C41657TandG4234CinnorthChineseHanpopulation.METHODS:Thesinglenucleotidepolymorphisms(SNPs)oftheC41657TandG4234CintheXRCC2geneallcelllungcancerpatientsand300healthycontrols.RESULTS:ThereorphisminnorthChineseHanpopulation.ThegenotypeandallelotypedistributionsoftheXRCC2C
4、41657TandG4234CSNPsinthenonsmallcelllungcancerpatientsthoseofthecontrols(allP0.05).gCl21.5mmol/L(C41657T)或1.25mmol/L(G4234C)，TaqDNA聚合酶2U，dNTPs160μmmol/L和上、下游引物各200nmol/L.PCR反应条件为：94℃5min预变性后，94℃变性45s,72℃(C41657T)或70.1℃(G4234C)退火45s,72℃延伸1min，35个循环后，72℃继续延伸7min.XRCC2C41657TPCR产物经限制性内切酶Mva
5、Ⅰ166.7nkat于37℃酶切16h后进行40g/L脂糖凝胶电泳.C/C基因型为315bp,42bp和59bp3个DNA片段，T/T基因型为357bp和59bp两个DNA片段，C/T基因型为315bp,357bp,42bp和59bp4个DNA片段(图1).XRCC2G4234CPCR产物经限制性内切酶MluⅠ166.7nkat于37℃酶切20h后，进行40g/L琼脂糖凝胶电泳.G/G基因型为251bp一个DNA片段，G/C基因型为251bp,230bp和21bp3个DNA片段，C/C基因型为21bp和230bp两个DNA片段(图2).为进行基因型检测的质量控制，每一批
6、PCR反应均以灭菌蒸馏水替代模板DNA作为阴性对照，实验样本随机抽取10%进行重复实验.图1PCRMvaⅠ酶切的XRCC2C41657TSNP基因分型略图2PCRMluⅠ酶切的XRCC2G4234CSNP基因分型略统计学分析：采用χ2检验对健康对照组XRCC2C41657T，G4234C多态位点的基因型分布进行HardycBrideS,etal.VariantsinDNAdoublestrandbreakrepairgenesandbreastcancersusceptibility［J］.HumMolGe,2002,11(12):1399-1407.［2］Miller
7、SA,DybesDD,PoleskyHF.AsimplesaltingoutprocedureforextractingDNAfromhumannucleatedcells［J］.NucleicAcidsRes,1988,16(3):1215.［3］CarrAM.Checkingthatreplicationbreakdoina［J］.Science,2002,297(5581):557-558.［4］DeansB,GriffinCS,OReganP,etal.Homologousrebinationdeficiencyleadstopr
