多发性骨髓瘤骨髓间充质干细胞生物学特性分析.doc

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1、多发性骨髓瘤骨髓间充质干细胞生物学特性分析作者：朱步东任军王湘漪李昕聂鎏【摘要】为了研究多发性骨髓瘤病人骨髓间充质干细胞的病理生物学特性，以探讨MSC在MM骨损伤发生中的作用，取11例初治丽病人和5例正常人骨髓，用贴壁法体外分离培养MSC。应用流式细胞术分析丽骨髓MSC表型，MTT法检测MSC增殖能力，组织化学染色测定细胞向成骨细胞和脂肪细胞分化能力。应用酶联免疫吸附实验测定细胞培养上清液中白介素-6和干细胞生长因子浓度。收集MSC培养上清作为条件培养液，按梯度浓度加入人SK0007骨髓瘤细胞系培养体系中，MTT法测定细胞增殖能力

2、差异。结果表明：与正常人骨髓MSC比较，丽患者骨髓MSC的表型无改变，均一表达CD29、CD73、CD166和HLA-ABC，不表达CD45和CD31；丽患者骨髓MSC增殖和分化能力正常，且具有相似的成骨和成脂肪分化功能；嫌1骨髓MSC分泌IL-6和SCF的水平均明显高于正常；匾来源的MSC培养上清显著促进SKOO07细胞的增殖。结论：丽患者骨髓MSC的增殖分化功能正常，画时骨损伤可能与MSC分化功能无关，而IL-6和SCF高表达为骨髓瘤细胞提供了必要的生存信号。【关键词】间充质干细胞；多发性骨髓瘤；细胞因子BiologicalP

3、ropertiesofMesenchymalStemCellsDerivedfromBonithMultipleMyelomaAbstractThestudywetheroleofmesencs)inthepathogenesticularlyobservediM),thebiologicalfevedMSCsfrompatientestigated.MarrowasedfromllnewlydiagMMand5normaladultedandculture-expantiesofadherencetophenotypewasan

4、alyzeechnique.TheprolifservedbyMTTassayanioncapacitiesintooblastswereassessedichistochemicalstationsofIL-6andSCFitantweredetectedbyosorbentassay(ELISeMarrowofPatientswaspurposedtoexplorhymalstemcells(MSCisofbonediseaseparnmultiplemyeloma(MaturesofmarrowderiswithMMhave

5、beeninvpirateswereharvestnosedpatientswithsandMSCswereisolatdedbythecellproperlasticflasks,ThepdbyflowcytometricterationofMSCswasobdthEirdifferentiatsteoblastsandadipowithlineage-specifining.Theconcentrantheculturesupernaenzyme-1inkedimmunA).MSCculturesupernatantswere

6、collectrformedtoevaluatetoliferationofanMMcelllineSK0007cellshatbonemarrowderintswerehomogenouslD73,CD166andHLA-ABatopoieticcellmarkalcellmarkerCD31,twassimilartothatoffromnormaladults.MatMSCsfromMMpatienoliferatedatsimilaatientsoccupiedinvdipogeniccapacityaults.Thele

7、velsofILupernatantweregreaMpatientsbyELISAasCculturesupernatandisplayedenhancedaeproliferationofSKludedthatmarrow-deedandMTTassaywaspehEIrsupportonthepr.TheresultsshowedtvedMSCsfromMMpatieypositiveforCD29,CCandnegativeforhemerCD45andendothelihephenotypeofwhichmarrowco

8、unterpartsTTassayindicatedthtsornormaladultsprrrates.MSCsfromMMpitroosteogenicandasthosefromnormalad-6andSCFinculturestlyup-