肠道多重耐药细菌感染动物模型的建立及益生菌干预的实验研究

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1、肠道多重耐药细菌感染动物模型的建立及益生菌干预的实验研究英文摘要sensitiveexperimentresultsofmultipledrugresistanceinpseudomonasaeruginosahavechanged,butthiswasnotstatisticallysignificant.3.TheresultsshowedthattheprobioticswasdosedependentwayinhibitingtheadhesionandinvasionofMDR-PAtoCaco-2cells.Theaggressivi

2、tyofMDR-PAdecreasedby86%whenprobioticsdoseforwascometo109CFU.DifferentdosesofMDR-PAandCaco-2cellswereincubation2h,usingflowcytometryinstrumenttesting,theresultsfoundthatMDR-PAcaninduceintestinalepithelialcellapoptosis,alongwiththeincreaseofthedoseofapoptosisrateincreasedgradu

3、ally.LPcaninhibittheMDR-PAtoCaco-2cellsinducedapoptosis,andtheinhibitioneffectofadhesioninhibitionmethodwasmoreapparentthancompetitiveexclusionmethod.4.AfterLPinterference,themultipleresistancequalityofpseudomonasaeruginosahaschanged.Comparedwithbefore,thediameterofdrugsuscep

4、tibilityinMEM,TZP,ATM-30andCAZ-30wereincreased,butthiswasnotstatisticallysignificant.CONCLUSION:1.Threemethodsofthisexperimentallcanbesuccessfullytoestablishanimalmodelwithintestinalinfectionbymultipledrugresistancepseudomonasaeruginosa,andeachmodelhasitsdifferentcharacterist

5、ics.2.Probioticscantreatdiarrheaofmiceintestinalinfectionbymultipledrugresistancepseudomonasaeruginosaandprotecttheintestinalbarrierfunction.3.ProbioticscaninhibittheadhesionandinvasionofMDR-PAtoIntestinalepithelialcells.4.Probioticscanweakentheresistanceofmultipledrugresista

6、nceinpseudomonasaeruginosaafterLPinterference,butthiswasnotstatisticallysignificant.【Keywords】:Probiotics;Lactobacillusplanetarium;MultipledrugV英文摘要肠道多重耐药细菌感染动物模型的建立及益生菌干预的实验研究resistance;Pseudomonasaeruginosa;Intestinalbarrier.WrittenbyHanhuaZhongSupervisedbyYangJunVI目录肠道多重耐药

7、细菌动物模型的建立及益生菌干预的实验研究.....................................1引言..............................................................................................................................1第一部分建立多重耐药铜绿假单胞菌肠道干染动物模型的比较和评价....................41材料和方法................................

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