土壤微生物与抗生素（英文ppt）organisms in soil and antibiotics课件

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1、SoilMicroorganismsandAntibioticsDecember6,2004KeniceFrank,AllisonJohnson,RubenKrantz,HannahWilburSoilbacteriaHabitats:WaterfilmsNeedwaterformetabolicprocessesOnsurfaceoforganicmatterNeedsurfaceonwhichtogrowInrhizosphereCompetitionCompetewithsurroundingbacteriaandfungiProdu

2、ctionofantibioticsbycompetingbacteriaSoilenvironmentsarehosttoagreatnumberofbacterialspeciesAntibioticsCanbeusedas:Fungicide,etcforfarmingAntibioticsforhumansandanimalsFromStrepomycesspeciesalone:500antibacterialproductsidentifiedObjectivesIdentifythroughlaboratorytestingm

3、embersofthesoilcommunitiesIsolateandcultivateantibacterialproducingbacteriafromsoilObserveandunderstandmembersofdifferentcommunitiesofsoilbacteriaSoilExperimentMethodsandMaterialsMethodsandMaterialsThefirststepofourexperimentwastochose3differentlocations/environments&toobt

4、ainsoilsamples.Wechoseariver,marshandforest.WethenusedsteriletubestoobtainthesampleRiverMarshForestMethodsandMaterialsWeaddedsterilizedH2Otoeachsoilsampleandstreaked500mlofeachsoiltypeonto4starch-caseinagarforeachsoiltype.Weincubatedtheseplatesatroomtemperature.MethodsandM

5、aterialsWeek2Wetook100mlofforestsampleanddiluteditwith400mlmoreofsterileH2OWeisolated4visuallydifferentcoloniesfromeachsoiltypeandstreakedeachcolonytypeontoindividualstarchcaseinagar-Wedidasmearoftheentireforestplateandtheentireriverplatebecausetherewerenoidentifiable,sepa

6、ratecoloniesIncubateatroomtemperatureMethodsandMaterialsWeek3Gotresultsfrompreviousweek’splates&examinedforanyStreptomycesbylookingforanyareasofinhibition(clearareassurroundingcolonies)Usingsoilplatepreparedforthegenerallab,wetriedtoisolateantibioticproducingorganismagainA

7、garplatesshowingareasofinhibitionMethodsandMaterialsWestreakedthelabspecimenandasamplefromeachsoilplatefromlastweekdownthemiddleofaBAP(bloodagarplate)andthenusedmethodfortestingsensitivitytoobserveforanyareasofinhibitionorhemolysisWethenstreakedS.aureusandE.colionstarchcas

8、inagarplatesperpendicularlytoacenterstreakofeachisolatedcolonytypewithouttouchingitBloodA