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ID:20545455
大小:8.54 MB
页数:26页
时间:2018-10-10
《土壤微生物与抗生素(英文ppt)organisms in soil and antibiotics课件》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、SoilMicroorganismsandAntibioticsDecember6,2004KeniceFrank,AllisonJohnson,RubenKrantz,HannahWilburSoilbacteriaHabitats:WaterfilmsNeedwaterformetabolicprocessesOnsurfaceoforganicmatterNeedsurfaceonwhichtogrowInrhizosphereCompetitionCompetewithsurroundingbacteriaandfungiProdu
2、ctionofantibioticsbycompetingbacteriaSoilenvironmentsarehosttoagreatnumberofbacterialspeciesAntibioticsCanbeusedas:Fungicide,etcforfarmingAntibioticsforhumansandanimalsFromStrepomycesspeciesalone:500antibacterialproductsidentifiedObjectivesIdentifythroughlaboratorytestingm
3、embersofthesoilcommunitiesIsolateandcultivateantibacterialproducingbacteriafromsoilObserveandunderstandmembersofdifferentcommunitiesofsoilbacteriaSoilExperimentMethodsandMaterialsMethodsandMaterialsThefirststepofourexperimentwastochose3differentlocations/environments&toobt
4、ainsoilsamples.Wechoseariver,marshandforest.WethenusedsteriletubestoobtainthesampleRiverMarshForestMethodsandMaterialsWeaddedsterilizedH2Otoeachsoilsampleandstreaked500mlofeachsoiltypeonto4starch-caseinagarforeachsoiltype.Weincubatedtheseplatesatroomtemperature.MethodsandM
5、aterialsWeek2Wetook100mlofforestsampleanddiluteditwith400mlmoreofsterileH2OWeisolated4visuallydifferentcoloniesfromeachsoiltypeandstreakedeachcolonytypeontoindividualstarchcaseinagar-Wedidasmearoftheentireforestplateandtheentireriverplatebecausetherewerenoidentifiable,sepa
6、ratecoloniesIncubateatroomtemperatureMethodsandMaterialsWeek3Gotresultsfrompreviousweek’splates&examinedforanyStreptomycesbylookingforanyareasofinhibition(clearareassurroundingcolonies)Usingsoilplatepreparedforthegenerallab,wetriedtoisolateantibioticproducingorganismagainA
7、garplatesshowingareasofinhibitionMethodsandMaterialsWestreakedthelabspecimenandasamplefromeachsoilplatefromlastweekdownthemiddleofaBAP(bloodagarplate)andthenusedmethodfortestingsensitivitytoobserveforanyareasofinhibitionorhemolysisWethenstreakedS.aureusandE.colionstarchcas
8、inagarplatesperpendicularlytoacenterstreakofeachisolatedcolonytypewithouttouchingitBloodA
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