抗生素英文课件精品kirbybauer

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1、Kirby-BauerTestTestingforAntibioticResistanceWhybothertesting?K-BTestisroutinelydonetomonitortheprevalenceofantibioticresistantbacteriaObserveforatrendinordertotakeprecautionarymeasuresForexample:developmentofnewdrugsdeterminingthemolecularbasisforresistanceandmodif

2、yexistingdrugsaccordinglyProceduresPrepareapureculture(18-24hrs)ofthesampleonanon-selectivemediumAdjustturbidityuntilitisequivalenttothe0.5McFarlandTurbidityStandard0.5McFarlandStandardSampleWithin15minutesofadjustingtheturbiditydipasterilecottonswabintothesamplestr

3、eakalawnofbacteriaonMueller-HintonagarLeavethelidagarfor3-5minutes(nomorethan15minutes)toallowplatetodryProceduresProceduresApplyantibioticimpregnateddisksonthebacteriallawnImportant:wherethediskdropsiswhereitstaysIncubatefor16-18hoursat33±2oCunlessotherwiseinstruct

4、edNote:1)FortestingStaphylococciwithoxacillinuseM-Hagarsupplementedwith2%NaClincubatefor24hoursat33±2oCFortestingStaphylococciwithvancomycinuseunsupplementedM-Hagarincubatefor24hoursat33±2oCResultsAntibioticsdiffuseoutontotheagarConcentrationofantibioticsdecreaseast

5、heydiffusefurtherawayfromthedisksAfterincubation,observeforaclearingonthebacteriallawn(zoneofinhibition)BacterialgrowthZoneofinhibitionincubationResultsMeasurethediametersofthezoneofinhibitionInterprettheresultsas“resistant”or“susceptible”accordingtotheguidelineprov

6、idedbytheNCCLSInterpretationofthezoneofinhibitionisdifferentforeachbacteria-antibioticcombinationE-test®SameprincipleastheKirby-BauerTestUsesaplasticstripwithapredefinedgradientofantibioticconcentrationResultsarereaddirectlyonthestripwherethezoneofinhibitionintersec

7、tswiththestripEZoneofinhibitionBacterialgrowthResultsInterpretresultsas“resistant”or“susceptible”accordingtotheguidelinesprovidedinthepackageinsertForambiguousresults,refertotheprovidedreadingguidefor:OrganismrelatedeffectsDrugrelatedeffectsResistancemechanismrelate

8、deffectsTechnicalandhandlingeffectsAPI®StaphTestIdentificationofthegeneraStaphylococcusMicrococcusKocuria20microtubuleswithdehydratedsubst