大多功能蛋白酶2基因与1型糖尿病易感性及发病年龄关系

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1、大多功能蛋白酶2基因与1型糖尿病易感性及发病年龄的关系大多功能蛋白酶2基因与1型糖尿病易感性及发病年龄的关系2010-12-15丁鹤林　程桦　傅祖植　邓庆丽　严励　　【摘要】　目的　研究大多功能蛋白酶(LMP)2基因与1型糖尿病(1-DM)及其发病年龄的关系。方法　71例1-DM患者及86名健康人参加，根据发病年龄将1-DM患者分为3组(≤14岁组，15～30岁组，≥31岁组)；采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术进行LMP2基因分型。结果　LMP2-R/R、LMP2-R/H、LMP2-H/H、LMP2-R及LM

2、P2-H频率在1-DM组分别为40.9%、53.5%、5.6%、67.6%、32.4%，在正常对照组分别为57.0%、32.6%、10.4%、73.3%、26.7%。LMP2-R/H频率在1-DM组显著高于对照组(P＜0.01)，而LMP2-R/R频率则显著低于对照组(P＜0.05)；LMP2各基因型及等位基因频率在1-DM各发病年龄组之间无显著性差异(P＞0.05)。结论　LMP2-R/H可能为1-DM的易感基因型，LMP2-R/H阳性者患1-DM的危险性增加；LMP2-R/R可能是1-DM的保护基因型，LMP2-R/R阳性者患1-DM

3、的危险性降低；LMP2各个基因型及等位基因与1-DM的发病年龄无关。　　【关键词】　糖尿病，1型　　基因　　聚合酶链反应　　限制性片段长度多态性RelationshipbetweenLMP2geneandsusceptibilitytotype-1diabetesmellitusandageofdiabeticonsetDINGHelin,CHENGHua,FUZuzhi,etal.DepartmentofEndocrinology,SunYat-senMemorialHospital,SunYat-senUniversityofMedi

4、calSciences,Guangzhou,510120　　【Abstract】　Objective　ToinvestigatetherelationshipbetweenLMP2geneandsusceptibilitytotype-1diabetesmellitusandageofdiabeticonset.Methods　TheLMP2genegenotypeswereidentifiedbypolymerasechainreaction-restrictionfragmentlengthpolymorphism(PCR-RFLP)

5、in71type-1diabetic(1-DM)patientsand86healthypersons(ascontrols).1-DMpatientsweredividedinto3groupsaccordingtoageofdiabeticonset,groupA≤14years,groupB15～30years,groupC≥31years.Results　ThefrequenciesofLMP2-R/R,LMP2-R/H,LMP2-H/H,LMP2-RandLMP2-Hwere　　40.9%,53.5%,5.6%,67.6%and

6、32.4%respectivelyin1-DMpatients,and57.0%,32.6%,10.4%,73.3%and26.7%respectivelyincontrols;Comparedwithcontrols,thefrequencyofLMP2-R/Hincreasedsignificantly(P＜0.01)whilethefrequencyofLMP2-R/Rdecreasedsignificantly(P＜0.05)inthe1-DMpatients.ThefrequenciesofLMP2geneshadnosigni

7、ficantdifferencebetweenagegroupsofdiabeticonset.Conclusions　TheremaybesignificantassociationofLMP2-R/HandLMP2-R/Rwiththesusceptibilityto1-DM.ThepersonswithLMP2-R/Hhaveahigherrisk,andthosewithLMP2-R/Radecreasedriskfor1-DM.TheremaybenoassociationofLMP2withtheageofonsetin1-D

8、M.　　【Keywords】　Diabetesmellitus,type1　　Gene　　Polymerasechainreaction　　Restrictionfragmentlengthp