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1、SELDI技术分析体外培养不同肝细胞株差异蛋白的表达(1) 作者：丁守怡，钱冬萌，牟文凤，闫志勇，宋旭霞，王斌 【关键词】表面增强激光解吸/离子化/飞行时间质谱；蛋白质陈列分析；肝肿瘤；肿瘤细胞,培养的；差异蛋白 AnalysisofdifferentproteinexpressionsindifferentlivercellstrainsinvitrousingSELDI 【Abstract】AIM:Toexaminethedifferentialexpressionsofproteinsinhepatomac
2、ellline,hepatomacelllinetransfectedbyHBV（）comparedwithnormallivercelllinebyusingsurfaceenhancedlaserdesorption/ionizationtimeofflightmassspectrometry,soastoestablishafoundationforfurtherstudyingonthemechanismofhepatomaattheproteinlevel.METHODS:The3typesofcellsabo
3、vementionedwereculturedasgeneralandcollectedwhentheywereingoodconditions.Aftercelldisruption,SELDITOFMSwasemployedtodetectthedifferentialexpressionsofproteomesofHepG2,HepGandLO2.RESULTS:NinetyoneproteinswerecapturedbyWCX2array.Comparedwithnormallivercelllines,int
4、hesegmentsfromMr5000to15000,proteinsintheHepG2andshowedapparentchanges,inwhich2proteinsexpressionswereincreasedincarcinomacells,theother5decreased.NineproteinsinHepG2wereincreasedand10proteinsinHepGwereincreased.CONCLUSION:TheSELDIProteinChiptechnologycanbeadesir
5、edmethodindetectingthebiologicalmarkersintheearlierperiodofhepatoma.Thismethodisofconvenience,highsensitivity,andgoodreproducibility.Thebiologicaltagsoftissuespecificproteinswefoundhaveapotentialapplyingvalueintheearlydiagnosisofhepatoma.Thesetagsarealsomeaningfu
6、linscreeningandidentifyingsignalproteinsfromserumandtissuespecimenindifferenttypesofhepatoma.Certainfoundationhasbeenestablishedinstudyingtheetiopathogenesisofhepatomaatthelevelofproteins,aswellasthesearchingofnewtherapeutictargetsites. 【Keywords】SELDITOFMS;prot
7、einarrayanalysis;liverneoplasms;tumorcells,cultured;distinctprotein 【摘要】目的：运用表面增强激光解吸/离子化/飞行时间质谱技术,检测体外培养的肝癌细胞株（HepG2）,转染乙肝病毒的肝癌细胞株（）与正常肝细胞株（LO2）蛋白质的差异表达，筛选肝细胞癌的标志蛋白，为进一步研究肝癌发病的蛋白质组学机制奠定基础.方法：常规培养上述3种细胞，细胞状态良好时收集细胞，裂解细胞后采用SELDITOFMS技术用WCX2芯片检测HepG2,,LO2细胞内蛋白质组学的
8、差异表达.结果：WCX2芯片共捕获91个蛋白，在Mr5000~15000区段，与正常肝细胞株比较，有7个蛋白质在两种肝癌细胞中出现明显变化，其中2个蛋白在肝癌细胞中表达量增高，5个蛋白在肝癌细胞中表达量降低；另外两种肝癌细胞株也有其各自的标志蛋白，9个蛋白分子在HepG2表达量增高，10个蛋白分子在表达量增高.结论：
