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1、THEJOURNALOFBIOLOGICALCHEMISTRYVol.260,No.30,IssueofDecember25,pp.16279-16285,198501985byTheAmericanSocietyofBiologicalChemists,Inc.PrintedinU.S.A.TheRoleofLinkProteininMediatingtheInteractionbetweenHyaluronicAcidandNewly-SecretedProteoglycanSubunitsfromAdultHumanArticularCartilage*(Receivedf
2、orpublication,March11,1985)LeeI.MelchingSandPeterJ.RoughleySglIFromthe$JointDiseasesLaboratory,ShrinersHospitalforCrippledChildren,Montreal,Quebec,CanadaH3GlA6andthe$DepartmentofExperimentalSurgery,McGillUniversity,Montreal,Quebec,CanadaH3AIA4Normaladulthumanarticularcartilageinorgancartilage
3、,thecollagennetworkpreventsthemoleculesfromculturesecretesproteoglycansubunitsthatcannotin-reachingtheirfullyextendedsize(Mowetal.,1984).Theitiallyinteractinanormalmannerwithhyaluronicosmoticpressureassociatedwiththissystemendowstheacidunlessthelatterispresentathighconcentrationscartilagewith
4、acapacitytogainorlosewaterwhenitsexternalandaneutralpHisemployed.However,ifthenewlyenvironmentisaltered,andsoallowsthetissuetofunctionsecretedsubunitisallowedtomatureinthecartilageinareversiblemannerinresistingcompressiveforces(Kemp-matrixforupto12h,thenitsabilitytointeractissonetal.,1976).Th
5、emaintenanceandlocalizationofanindistinguishablefromthatofitsmorematurecoun-intactaggregateareimportantinthisrole.terparts.ThisconversiondoesnottakeplaceiftheTheprocessof.aggregationhasbeenstudiedinseveralproteoglycansubunitsareincubatedindilutesolutionssystems.Intheratchondrosarcoma,newlysyn
6、thesizedpro-intheabsenceofthecartilage,anditispreventedbyculturingatlowtemperature.Thenewlysecretedpro-teoglycanshavebeenshowntoberapidlyincorporatedintoteoglycansubunitscan,however,beinducedtointeractlinkprotein-stabilizedaggregates(Kimuraetal.,1979).Inthewithhyaluronicacidbythepresenceoflin
7、kproteins.samesystemithasbeendemonstratedthattheintracellularThecomplexformedbythesethreecomponentscannotpoolofcoreprotein,priortotheadditionoftheglycosami-bedissociatedinthepresenceofhyaluronicacidoli-noglycanchains,isalsocapableofformingsta
