星点设计效应面法优选人参叶酶法提取工艺

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1、万方数据第12卷第4期21306年4月中国实验方剂学杂志ChineseJoumalofExperimentalTraditionalMedicalFormulaeV01．12．NO4Apr．，2006星点设计一效应面法优选人参叶酶法提取工艺昊清‘，倪宁，王玉蓉，吴华(北京中医药大学中药学院，北京100102)[摘薹】目的：通过星点设计墩应面法优选人参叶酶法提取工艺。方法：用纤维素酶法从人参叶中提取人参总皂苷，并用星点设计．效应曲面分析方法进行多元线性回归和二项式拟合以分析酶反应参数(包括pH值，加水量

2、，酶底比)对人参总皂苷得率的影响，从中选择最佳工艺，进行预测分析，得出预测值并与实际值比较。结果：二项式拟合复合相关系数较高，r为O．940．最佳提取工艺：DH为4．8，加水量为24倍，酶底比为40U／g，人参皂苷提取率实际值为6．29％，预测值为6．37％。结论：实际值和预测值吻合度高．星点设计．效应面法优选人参总皂苷酶法提取工艺是成功的。[关键词]人参总皂苷；星点设计．效应面法；提取工艺；人参叶；纤维索酵；[中围分类号】R2836[文献标识码】B[文章编号】1005-9903(2006}04—00

3、05—04jOptimizationofCellulaseHydrolysisExtractionProcessofFoHumGinsengbyCentralCompositeDesign／ResponseSurfaceMethodologyWUQing。，NINing，WANGYu-rong，时Hua(BeltingUniversityofChiac．seMedicine，Belting100102，China)[AlK'lraet]Objective：Toobtaintheoptimalcellu

4、lasehydrolysisextractionprocessofFohumginsengbyCentralCompositeDesign／ResponseSurfaceMethodology．Methods：Toextractginsenosidesfromfoliumginsengbyaddingcellulasedigestionprocess．Theparametersofenzymehydrolysisactionisdividedintoindependentvariablesinclud

5、ingpHvalue，watervolume，theratioofenzyme／theamountofherbal，anddependentvariables—theextractionratesofginsenosides，LinearornonlinearmathematicmodelswerfjusedtOestimatetherelationshipbetweenindependentanddependentvariables．Theresponsesurfacemethodologywasu

6、sedtooptimizetheconditionsofextraction．PredictionWaScarriedoutthroughcomparingtheobservedandpredictedvalues．Results：Itwasshowedthatthecoefficientofcorrelationofseeond-orderquadraticmodelwashi曲．necorrelationCoefficientreached0．940．neoptimumCOnditionsofex

7、tractionwereobtained．TheoptimumconditionW08pH4．8，24timesamountofwatermorethanmaterialmedica，andenzyme／substratewas40U／g．Theobservedvaluesandthepredicatedoneofextractionratiow鹏6．29％and6．37％．Condusion：Theobservedvaluesandpredictedoneateclosetoeachother，wh

8、ichprovethattheoptimizationofeeUulasedigestionextractionprocessofginsenosidesfromfoliumginsengbyCentralCompositeDesign／ResponseSurfaceMethodologyisl_easoHableandSucce88hil．[Keywords]ginsenosides；CentralCompositeDesign／ResponseSur