碱性成纤维细胞生长因子与瘢痕成纤维细胞ⅰ、ⅲ型胶原蛋白的代谢

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1、中国组织工程研究与临床康复74拳匆。47』2010—10—08出版JournalofClinicalRehabilitativeTissueEngineeringResearchOctober82010VoL14，No．47碱性成纤维细胞生长因子与瘢痕成纤维细胞I、III型胶原蛋白的代谢☆谢举临’，卞徽宁，李厚东’，舒斌’，祁少海’，唐锦明’，徐盈斌’，利天增’，刘旭盛’BasicfibroblastgrowthfactorandmetabolismofIandⅢcollagenproteininscarfibr

2、oblastsxieJu-lin’．BianHui．ning，LiHou-dong’，ShuBin’，QiShao—hal’，TangJin—ming’．XuYing-bin’，LiTian—zeng，LiuXu—shengAbstractDepartmentofBurn，BACKGROUND：Studieshaveconfirmedthatbasicfibroblastgrowthfactor(bFGF)canpromotewoundsurfacehealing．FirsIAffiIiateclScholars

3、havepaidgreata~enfiononwhetherbFGFcaninducefibroblasticproliferationandleadloscarhyperplasiadudngHospita1．SunYat-SenUniversity．woundsudacehealing．GuangzhouOBJECTlVE：ToinvestigatetheregulatoryefectsofbFGFonthesynthesisanddegradationoffibroblastIandlIIcollagen5

4、10080，Guangdongprotein．Province，China；METHODS：HyperplasticscarUssuewasobtainedfrompatientsundergoingscarplastyattheDepa~mentofBurn．FirstAmliatedDepartmentofBurn．Hospital，SunYat．SenUniversity．Fibroblastsofscartissuewereculturedbytissueblockmethod．Thesecondpass

5、ageofcellsGuangdongPeople’sHospital，Guangzhouwascollected．EfectsofbFGF(0—500pg／L)onsynthesisandsecretionofIandIIcollagenproteinandcelImatrix510080．Guangdongmetaloproteinase1inscar-derivedfibroblastsweremeasuredbychloraminesTRT-PCRandWesternblotassay．Province，

6、ChinaRESULTSANDCONCLUSION：bFGFstimulationhadnoefectonhydroxyproline．IandlIIcollagenproteinmRNAexpression．LowmassconcentrationofbFGFdidnotafectcelImatrixmetalloproteinase1expression．butcellmatrixmetalloproteinase1XieJu．☆Doctor,expressionwasincreasedwithincreas

7、edmassconcentrationofbFGF，especialy50，10O．500pglLgroups(P<0．05orP<0．01)．，、ssociateprofessor,Simultaneously．changesincelImatfixmetalloproteinase1expressionwereidenticalincellsandsupernatant．ResultshaveDepartmentofBurn，FirstAfiIiatedsuggestedthathighmassconcent

8、rationofbFGFcontributestodegradationofcolagenproteinbyincreasingcellmatrixHospitaI。Sunmetaloproteinase1synthesis．resultinginavoidingexcessivedepositionofextracellularmatrixYat-SenUniversi